xMAP 实验策略及优化：肺炎链球菌相关应用

微球平台结合单克隆抗体进行 13 种血清型多重鉴定

Development and validation of a sensitive and robust multiplex antigen capture assay to quantify Streptococcus pneumoniae serotype-specific capsular polysaccharides in urine 
mSphere. 2022 Aug 1;e0011422. doi: 10.1128/msphere.00114-22

使用微球法对肺炎儿童尿液样本进行抗原检测

Application of a Pneumococcal serotype-specific urinary antigen detection test for identification of pediatric pneumonia in Burkina Faso

Pediatr Infect Dis J. 2021 May 1;40(5):418-425

建立覆盖 11 种肺炎链球菌血清型的微球尿抗原检测法

Qualification and clinical validation of an immunodiagnostic assay for detecting 11 additional Streptococcus pneumoniae serotype-specific polysaccharides in human urine

Clin Infect Dis 2020;71:E430–8. 10.1093/cid/ciaa158

在前瞻性研究中使用微球多重法确认由疫苗覆盖血清型引发的成人肺炎链球菌病比例
Pneumococcal epidemiology among US adults hospitalized for community-acquired pneumonia
Vaccine. 2019 May 31;37(25):3352-3361

针对 24 种血清型及交叉反应血清型建立微球尿抗原多重检测法
Development of an extended-specificity multiplex immunoassay for detection of Streptococcus pneumoniae serotype-specific antigen in urine by use of human monoclonal antibodies
Clin Vaccine Immunol. 2017 Dec 5;24(12):e00262-17

开发同时检测 13 种肺炎链球菌血清型的多重检测法

Validation of an immunodiagnostic assay for detection of 13 Streptococcus pneumoniae serotype-specific polysaccharides in human urine
Clin Vaccine Immunol. 2012 Aug;19(8):1131-41

利用微球平台以免疫及分子组合检测法对肺炎链球菌进行分型鉴定

Development of an automated and multiplexed serotyping assay for Streptococcus pneumoniae
Clin Vaccine Immunol. 2011 Nov; 18(11): 1900–1907

以多重微球法评估营养状态及接种肺炎链球菌疫苗后抗体效价间的关系

Dietary intake and pneumococcal vaccine response among children (5-7 years) in Msambweni division, Kwale County, Kenya

mSphere. 2022 Aug Front Nutr. 2022 May 23;9:830294

Factors influencing PCV13 specific antibody response in Danish children starting in day care

Sci Rep. 2020 Apr 10;10(1):6179

以多重微球法评估血液病不同基因型及肺炎链球菌疫苗免疫反应差异

Comparison of pneumococcal vaccination response in children with sickle cell disease: HbSS and HbSC

Allergol Immunopathol (Madr). 2019 Nov-Dec;47(6):564-569

验证肺炎链球菌疫苗抗体微球检测法与 WHO ELISA 法的等效性

Evaluation of a validated Luminex-based multiplex immunoassay for measuring immunoglobulin G antibodies in serum to pneumococcal capsular polysaccharides

mSphere. 2018 Aug 8;3(4):e00127-18

建立基于微球平台的 13 价肺炎链球菌疫苗抗体效价检测法

Development and validation of 13-plex Luminex-based assay for measuring human serum antibodies to Streptococcus pneumoniae capsular polysaccharides

mSphere. 2018 Aug 8;3(4):e00128-18

比较三种肺炎链球菌疫苗抗体检测法（微球法及两种免疫 ELISA 法）

Interlaboratory comparison of three multiplexed bead-based immunoassays for measuring serum antibodies to pneumococcal polysaccharides

Clin Vaccine Immunol. 2010 May;17(5):862-9

建立并优化 13 种肺炎链球菌血清型抗体检测法

Optimization and application of a multiplex bead-based assay to quantify serotype-specific IgG against Streptococcus pneumoniae polysaccharides: response to the booster vaccine after immunization with the pneumococcal 7-valent conjugate vaccine

Clin Vaccine Immunol. 2010 Apr;17(4):674-82

通过 DMTMM 修饰肺炎链球菌多糖抗原以与 Luminex 微球进行偶联

A novel chemistry for conjugating pneumococcal polysaccharides to Luminex microspheres

J Immunol Methods. 2006 Feb 20;309(1-2):75-85

建立同时定量 9 种肺炎链球菌血清型抗体的多重微球法

Development and validation of a nonaplex assay for the simultaneous quantitation of antibodies to nine Streptococcus pneumoniae serotypes

J Immunol Methods. 2005 Jan;296(1-2):135-47