Preface
Organoids, as three-dimensional cell culture models, have demonstrated tremendous potential across diverse fields including disease research, drug development, precision medicine, and regenerative medicine. Accurate and efficient analysis of organoid viability is essential for various experiments such as organoid culture and drug screening. However, conventional viability assays like CCK-8 and MTT exhibit critical limitations when applied to organoids’ complex 3D structures. These methods are prone to interference from subtle environmental fluctuations, often resulting in inadequate sensitivity and cumbersome procedures. Meanwhile, ATP measurement requires the lysis of organoid cells, preventing real-time monitoring of live-cell viability and leading to either discontinuous analysis or unnecessary sample waste.
Say NO to Organoid Heartbreak:
A Smart Combo to Keep Your Precious Organoid Thriving
bioGenous has recently launched its LivingCell-Fluo Insight Real-time Organoid Viability Analyzer. When used together with the matching
LiveCell-FluoTM Organoid Viability Assay Kit (#E238004), this system provides a complete solution to address your concerns about organoid viability.
Product Information
LivingCell-Fluo Insight
Unparalleled Precision: Detect Microscopic Changes with Confidence
Equipped with high-precision sensors, our system delivers ultra-sensitive detection of organoid samples with ≤0.4% measurement error.
The bottom-reading design eliminates top-layer interference while maintaining a closed, contamination-free environment - no lid removal required.
Intuitive Workflow: Maximize Productivity Without Sacrificing Accuracy
Ready-to-use with streamlined operation: simply load samples for immediate, non-destructive analysis.
96-well plate scans in <60 seconds, dramatically boosting throughput without compromising data quality.
Future-Ready Design: Seamlessly Scale for Evolving Needs
Compatible with 96-well and other standard microplates
Maintain post-assay cultures under sterile conditions for continued expansion
Robotic arm interfaces for seamless integration into automated drug screening platforms
Product Introduction
Instant Analysis: Three Steps, Two Minutes, One Plug-and-Play System
From raw data to actionable insights - streamline your entire analysis workflow with:
·Professional-grade pharmacodynamics tools
·Seamless LIMS integration
·Future-proof data management
·Compatible with major-brand 96/384-well plates
·Adjustable scan speed
·Custom detection pathways
·Dynamic gain control
Case Study
Application of LivingCell-Fluo Insight in Precision Assessment of Colorectal Cancer Organoid Models
By integrating our LivingCell-Fluo™ Organoid Viability Assay Kit (#E238004), bioGenous customers obtained the coefficient of variation (CV) for data reproducibility. A comparison of instrument precision through frequency deviation distribution plots demonstrated that LivingCell-Fluo Insight exhibits outstanding performance in data precision and consistency. Moreover, its sensitivity can accurately distinguish minute changes in organoid viability, reliably detecting even 2% deviations. This high-sensitivity detection capability significantly improves data quality and reliability, yielding more precise experimental results.
*This data is derived from customer experiments.
Application of LivingCell-Fluo Insight in 5-Fluorouracil Drug Screening
Using the Organoid Bioprinter high-throughput 3D gel printing system, pancreatic ductal adenocarcinoma organoids and colorectal cancer organoids were precisely inoculated in 96-well plates. After treating the organoids with different concentrations of 5-fluorouracil, the Star-Map F1 organoid imaging system was used to perform three-dimensional imaging observation of the organoids in the 96-well plates. The results showed that the drug had significant cytotoxic effects on the cells. Subsequently, the LivingCell-Fluo Insight real-time organoid viability analyzer was used to detect cell viability in the 96-well plate samples. The software's one-click curve fitting function quickly generated IC50 values (PDC=0.8526μM, CRC=1.190μM) and automatically exported a complete experimental report.
Application of LivingCell-Fluo Insight in Monitoring Human Cholangiocyte Organoid Growth
During the cultivation and biobanking of organoids, monitoring their viability and growth status is crucial. We employed LivingCell-Fluo Insight to perform consecutive viability assessments of human cholangiocyte organoids at two-day intervals, enabling evaluation of their growth status and continuous monitoring. The LivingCell-Fluo Insight real-time organoid viability analyzer data processing system can automatically calculate growth rates across different detection periods with one-click operation, facilitating in-depth analysis of cellular growth conditions.
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