经上世纪四十年代美国科学家Esmond Emerson Snell在生物代谢的研究过程中，从鸡蛋清中发现亲和素(Avidin)以来，科学家和研究人员们陆续发现源自链霉菌的链霉亲和素（Streptavidin,SA）并通过基因及生物工程技术实现了多种类型的重组蛋白。链霉亲和素作为由4个相同亚基组成的同源四聚体蛋白，其每单个亚基都可以特异性结合单个生物素，这种紧密而特异的结合非常快速，也是目前已知最强的非共价相互作用之一。
高亲和力和四个亚基的级联放大效应，以及这种特异结合还能够承受极端的 pH 值、温度、有机溶剂和变性试剂等环境条件，使得链霉亲和素-生物素系统被广泛应用于分子生物学为基础的检测、医疗诊断、偶联标记、亲和纯化、药物传递和生物传感等众多的方面和领域中。
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高品质链霉亲和素源自罗氏
罗氏CustomBiotech率先开发了重组链霉亲和素，目前是世界上最大的制造商之一。
作为专业的高品质体外诊断和生物技术工业原料生产商，持续通过产品创新和严苛的质量控制引领行业标准。
1、更牢固的生物素结合，提升体系捕获和结合质量
高亲和力 KD:10-15 mol/L
2、单位质量含有更多的活性链霉亲和素，可结合更多的生物素。提升捕获，放大效率
高生物素结合
High biotin-binding capacity (≥17 U/mg protein)
3、更高的安全性，提升反应体系质量和稳定性
无蛋白酶 Protease-free (≤0.001 U/mg)
因多年来专业的生产和应用开发经验以及生产规模，来自罗氏CustomBiotech的链霉亲和素产品系列已在全球范围赢得众多合作伙伴和机构的长期青睐。
易被忽视的“链霉亲和素抗体”干扰
免疫检测会受到不同类型的生物样本內源性的干扰。例如，熟知的抗异嗜性抗体(HAMA)、类风湿因子(RF) 等。随着链霉亲和素-生物素系统的广泛运用，现实中常常被忽视的是，样本中内源性的抗链霉亲和素抗体 (Anti-streptavidin antibody, ASA) 或者可能的链霉亲和素结合蛋白可能会对基于含有链霉素亲和素的检测体系带来干扰，导致偶联、检测或者诊断结果的准确性受到影响。研究发现抗链霉亲和素抗体干扰的可能机制是，样本中的ASA与反应体系中的SA结合，影响特异性抗原-抗体正确体系的构建：
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链霉亲和素抗体ASA干扰消除
为此研究和开发人员曾尝试通过载有SA的固相介质诸如微孔板、磁微粒对样本进行预处理，尽可能预结合样本中的“链霉亲和素抗体”以期达到ASA消除效果，但是因该方法需要额外的试剂耗材和处理，以及潜在的样本损失和污染风险不可避免地对检测成本、效率带来影响。
罗氏 CustomBiotech 践行持续的诊断原料产品创新， 开发和工业化生产提供ASA特异性干扰消除产品--非活性链霉亲和素突变体(Streptavidin rec. inactive, poly) 无需额外步骤仅需添加至现行体系中，轻松实现特异性阻断和消除来自检测样本中ASA干扰，提升检测系统的准确型和特异性。
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关于罗氏诊断工业原料部门
Roche CustomBiotech
罗氏诊断生命科学部工业原料全球总部位于德国Penzberg，作为欧洲主要的生物工程制剂生产商在世界范围内为体外诊断和生物科技公司提供一站式体外诊断产品原料。秉承几十年来先进、创新和高质量的研发以及生产理念，提供涵盖生化、免疫、分子体外诊断检测以及生物医药等各应用领域的高品质全系列工业原料产品。
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2026年CACLP 罗氏诊断
精彩活动预览
仅供进一步加工处理工业使用
MC-CN-05675，有效期至2028年2月2日
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