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合适的对照或方法，让你的科研结果去伪存真

合适的对照或方法，让你的科研结果去伪存真 基因快讯
2021-03-15
6
导读：抗体的高特异性、高灵敏度和可重复性非常重要，而合理的对照也不可或缺。论对照的重要性，谁用谁知道！
抗体
的高特异性、高灵敏度和可重复性非常重要，而合理的对照也不可或缺。在实验操作步骤中添加阳性和阴性对照可确保分析正常进行且结果可靠。条理清晰证据充分的数据更让审稿人一目了然且易接受，让你的科研结果去伪存真，把自己的科研故事讲的更动听。


<免疫印迹（WB）阳性对照>

不是自己生产且没有严格验证的抗体你敢用吗？经常做不出来浪费珍贵的样品、浪费自己的时间不要紧吗？！

CST自己生产所有抗体并进行严格验证，以下这些阳性和阴性细胞裂解液和蛋白对照是在CST实验室生产并用于抗体验证和批次间测试的。我们推荐你在WB中使用这些裂解物作为对照，来保证合适地进行WB实验。使用合适的对照同样方便了实验问题的诊断，可以很快找到究竟是WB的步骤还是样品出了问题。

细胞裂解液对照

No. Name
No. Name
11972 LC3 Control Cell Extracts
12052 Smad2/3 Control Cell Extracts
34499 p70 S6 Kinase MCF7 Control Cell Extracts
51367 4E-BP1 Control Cell Extracts
5634 EGF Receptor Control Cell Extracts
7988 PathScan® Sandwich ELISA Control Phospho Cell Extract I
7989 PathScan® Sandwich ELISA Control Phospho Cell Extract II
9133 Stat3 Control Cell Extracts
9158 AMPK Control Cell Extracts
9160 MEK1/2 HeLa Control Cell Extracts
9173 Stat1 Control Cell Extracts
9193 CREB Control Cell Extracts
9194 p44/42 MAPK (Erk1/2) Control Cell Extracts
9213 p38 MAPK Control Cell Extracts
9223 ATF-2 Control Cell Extracts
9233 MKK3/MKK6 Control Cell Extracts
9243 NF-κB Control Cell Extracts
9253 SAPK/JNK Control Cell Extracts
9263 c-Jun Control Cell Extracts
9273 Akt Control Cell Extracts
9353 Stat5 Control Cell Extracts
9663 Caspase-3 Control Cell Extracts
9852 Chaps Cell Extract Buffer (10X)

蛋白对照

No. Name
No. Name
2904 VEGF Receptor 2 Control Proteins
9183 Elk-1 Control Proteins
9293 Bad Control Proteins
9303 Rb Control Proteins

更有阳性对照总表，汇总了活化状态特异性抗体的阳性对照——细胞系和处理，同时也可作为恰当地控制实验的参考。

如图所示：

Antibody Cell Line Treatment Control Extract
Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb #9606 Jurkat Calyculin A (100 nM for 30 min) Akt Control Cell Extracts #9273
Phospho-(Ser) 14-3-3 Binding Motif Antibody #9601 Jurkat Calyculin A (100 nM for 30 min) Akt Control Cell Extracts #9273
………… ………… ………… …………

这个表中提到的处理方法，可以用作活化状态特异性抗体的阳性对照，应该被看作是问题诊断的起始点。但是有些蛋白可能只是在特定模型中或特定的刺激中表达，所以这些蛋白可能没有合适的对照，您可以参考说明书中提供的图片上的细胞系来做对照。需要更多信息，请查看文末左下角的二维码或查看网址http://www.cst-c.com.cn/tools/Controls_Table.html。


<免疫沉淀（IP）对照>

同型对照抗体是用来判断由于Fc受体结合或其他蛋白间相互作用引起的一抗的非特异性结合。同型对照抗体应该与受试抗体有相同的免疫球蛋白类型。

同型对照

No. Name Applications
8726 Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate)  IP
2729 Normal Rabbit IgG1 IP, ChIP
No. Name Applications
5873 Mouse IgG1 (Magnetic Bead Conjugate) IP
5415 Mouse (G3A1) mAb IgG1 Isotype Control IP, IHC-P, IF-F, IF-IC, F, ChIP
4097 Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) IP, F
3420 Mouse IgG (Sepharose Bead Conjugate) IP
3900 Rabbit (DA1E) mAb IgG XP® Isotype Control IP, IHC-P, IF-IC, F, ChIP
4096 Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) IP, F
3423 Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) IP


<免疫组化（IHC）对照>

我们提供对照试剂来帮助你验证IHC分析的特异性和可信性。CST科学家开发SignalSlide®IHC对照作为评估染色试剂和方法的性能的重要工具。SignalSlide®IHC对照包含福尔马林固定，石蜡包埋的细胞颗粒作为IHC实验中的对照。每张片子包含一个阳性细胞团块，一个阴性细胞团块，每个包装有五张片子。

SignalSlide® IHC对照

No. Name To be used with Antibodies
No. Name To Be Used With Antibodies
8104 SignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls

9664,9661,9662,2035,9541
8101 SignalSlide® Phospho-Akt (Ser473) IHC Controls

2855,9644,4060,3787,2938,4691,4685,2920,9323,5482,5196,2997,2691,4858,4857,2211,5364,2217,2317
8102 SignalSlide® Phospho-EGF Receptor IHC Controls

2235,2237,3777,2236,2234,4404,4407,4267,9411,9417,9416
8117 SignalSlide® Phospho-ErbB Family IHC Controls

3777,4407,4267,2243,4290,2165,2242,4791
8118 SignalSlide® Phospho-Met (Tyr1234/1235) IHC Controls 3077
8103 SignalSlide® Phospho-p44/42 MAPK (Thr202/Tyr204) IHC Controls

4370,4376,4695,4696,9102,9108
8105 SignalSlide® Phospho-Stat1/3/5 IHC Controls

9167,9175,9145,4113,4904,9139,9314,9359
8106 SignalSlide® PTEN IHC Controls 9188,9559

同型对照

No. Name Applications
No. Name Applications
5415 Mouse (G3A1) mAb IgG1 Isotype Control IHC-P, IP, IF-F, IF-IC, F, ChIP
3900 Rabbit (DA1E) mAb IgG XP® Isotype Control IHC-P, IP, IF-IC, F, ChIP


<染色质免疫沉淀（ChIP）对照>

同型对照抗体是用来判断由于Fc受体结合或其他蛋白间相互作用引起的一抗的非特异性结合。同型对照抗体应该与受试抗体有相同的免疫球蛋白类型。

同型对照

No. Name Applications
No. Name Applications
2729 Normal Rabbit IgG1 ChIP, IP
5415 Mouse (G3A1) mAb IgG1 Isotype Control ChIP, IP, IHC-P, IF-F, IF-IC, F
3900 Rabbit (DA1E) mAb IgG XP® Isotype Control ChIP, IP, IHC-P, IF-IC, F


<IF的对照>

同型对照抗体是用来判断由于Fc受体结合或其他蛋白间相互作用引起的一抗的非特异性结合。同型对照抗体应该与受试抗体有相同的免疫球蛋白类型。

同型对照

No. Name Applications
No. Name Applications
8527 Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 594 Conjugate) IF-IC
8760 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 594Conjugate) IF-IC
9641 Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 555 Conjugate) IF-IC
5415 Mouse (G3A1) mAb IgG1 Isotype Control IF-F, IF-IC, IP, IHC-P, F, ChIP
3969 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 555 Conjugate) IF-IC
2975 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) IF-IC, F
2985 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) IF-IC, F
3900 Rabbit (DA1E) mAb IgG XP® Isotype Control IF-IC, F, IP, IHC-P, ChIP


<Flow的对照>

同型对照抗体是用来判断由于Fc受体结合或其他蛋白间相互作用引起的一抗的非特异性结合。同型对照抗体应该与受试抗体有相同的免疫球蛋白类型。

同型对照

No. Name Applications
9078 Rabbit (DA1E) mAb IgG XP® Isotype Control (Pacific Blue™ Conjugate) F
No. Name Applications
6899 Mouse (G3A1) mAb IgG1 Isotype Control (PE Conjugate) F
4843 Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 647 Conjugate) F
14411 Mouse (G3A1) mAb IgG1 Isotype Control (Pacific Blue™ Conjugate) F
5415 Mouse (G3A1) mAb IgG1 Isotype Control F, IP, IHC-P, IF-F, IF-IC,ChIP
4097 Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) F, IP
4878 Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) F
2985 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) F, IF-IC
4096 Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) F, IP
3900 Rabbit (DA1E) mAb IgG XP® Isotype Control F, IP, IHC-P, IF-IC, ChIP
2975 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) F, IF-IC
5742 Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) F
4340 Rabbit IgG Isotype Control (Alexa Fluor® 488 Conjugate) F
3452 Rabbit IgG Isotype Control (Alexa Fluor® 647 Conjugate) F


<PathScan® ELISA 细胞裂解物对照>

我们提供合适的ELISA阳性和阴性裂解物对照，可以将从不同分析板获得的信号标准化。


No. Name
No. Name
7988 PathScan® Sandwich ELISA Control Phospho Cell Extract I
7989 PathScan® Sandwich ELISA Control Phospho Cell Extract II


<PTMScan® 对照肽段>

我们提供合适的PTMScan酶消化对照肽段，可以作为质控阳参，确保质谱系统批次间性能稳定的标准更确保你实验的准确性。


No. Name
No. Name
12148 PTMScan® Lys-C Digested Control Peptides I
12219 PTMScan® Trypsin Digested Control Peptides II


<上样对照抗体>Western/IP辅助产品

上样对照抗体用于确保各泳道有相等的上样量，在WB结果用于定性或定量比较时非常重要。常用的上样对照抗体是在多种细胞系和组织中表达丰富的蛋白，包括例如β-Actin，COX IV，GAPDH，Histone H3，和α- and β-Tubulin等。

 小包装上样对照抗体套装

No. Name
No. Name
4670 Loading Control Antibody Sampler Kit (HRP Conjugate)
5142 Loading Control Antibody Sampler Kit
9774 Loading Control Antibody Sampler Kit (Mouse)

 上样对照抗体

No. Name Applications Reactivity
No. Name Applications Reactivity
12620 β-Actin (D6A8) Rabbit mAb (HRP Conjugate) W H, M, R, Mk, (Hm, C)
12748 Pan-Actin (D18C11) Rabbit mAb (HRP Conjugate) W H, M, R, Mk
4970 β-Actin (13E5) Rabbit mAb W, IHC-P, IHC-F, IF-IC, F H, M, R, Mk, B, Pg, (Hm, C, Dg, Hr)
5125 β-Actin (13E5) Rabbit mAb (HRP Conjugate) W H, M, R, Mk, B, Pg, (C, Hr)
12262 β-Actin (8H10D10) Mouse mAb (HRP Conjugate) W H, M, R, Hm, Mk, Dg
3700 β-Actin (8H10D10) Mouse mAb W, IHC-P, IF-IC, F H, M, R, Hm, Mk, Dg
4967 β-Actin Antibody W H, M, R, Hm, Mk, Mi, Dm, Z, B, (C, X, Dg, Pg, Hr)
5247 COX IV (3E11) Rabbit mAb (HRP Conjugate) W H, Mk
4850 COX IV (3E11) Rabbit mAb W, IP, IHC-P, IHC-F, IF-IC, F H, R, Mk, Z, B, Pg
4844 COX IV Antibody W, IP, IHC-P, F H, M, R, Mk, B
5174 GAPDH (D16H11) XP® Rabbit mAb W, IHC-P, IF-IC H, M, R, Mk, (Pg)
8884 GAPDH (D16H11) XP® Rabbit mAb (HRP Conjugate) W H, M, R, Mk
3683 GAPDH (14C10) Rabbit mAb (HRP Conjugate) W H, M, R, Mk
5014 GAPDH (14C10) Rabbit mAb (Biotinylated) W, IF-IC, F H, M, R, Mk
2118 GAPDH (14C10) Rabbit mAb W, IHC-P, IF-IC, F H, M, R, Mk, (Pg)
4499 Histone H3 (D1H2) XP® Rabbit mAb W, IHC-P, IF-IC H, M, R, Mk, (Hm, C, Dm, X, Z, B)
9717 Histone H3 (3H1) Rabbit mAb W H, M, R, Hm, Mk, Dm, Z, B, Dg, Pg
5192 Histone H3 (3H1) Rabbit mAb (HRP Conjugate) W H, M, R, Hm, Mk, Z, B, Pg
3638 Histone H3 (96C10) Mouse mAb W H, M, R, Mk, (Dm, X, Hr)
9715 Histone H3 Antibody W H, M, R, Mk, Z, B, Pg, (Dm)
5346 β-Tubulin (9F3) Rabbit mAb (HRP Conjugate) W H, M, R, Mk, Z, B, (C)
2128 β-Tubulin (9F3) Rabbit mAb W, IHC-P, IF-IC, F H, M, R, Mk, Z, B, (C)
2146 β-Tubulin Antibody W, IHC-P, IF-IC, F H, M, R, Mk, Z, B, (X)
2148 α/β-Tubulin Antibody W, IHC-P, IF-IC, F H, M, R, Mk, Z, B
2125 α-Tubulin (11H10) Rabbit mAb W, IHC-P, IF-IC, F H, M, R, Mk, Dm, B, (Dg, Pg)
9099 α-Tubulin (11H10) Rabbit mAb (HRP Conjugate) W H, M, R, Mk, Dm, B, (Dg)
12351 α-Tubulin (DM1A) Mouse mAb (HRP Conjugate) W H, M, R, Mk
3873 α-Tubulin (DM1A) Mouse mAb W, IHC-P, IF-IC H, M, R, Mk
2144 α-Tubulin Antibody W, IHC-P, IF-IC, F H, M, R, Mk, Dm, B, (X)

< 封闭肽 / 阻断肽 >WB/IHC辅助产品

封闭肽特异性结合它们所对应的抗体，阻止进一步的抗体结合，来证实抗体的特异性并消除非特异性结合的困扰。

封闭肽

No. Name
No. Name
1000 Phospho-Histone H3 (Ser10) Blocking Peptide
1002 β-Catenin Blocking Peptide
1004 HP1α Blocking Peptide
1005 Neurofilament-L Blocking Peptide
1007 TCF1 Blocking Peptide
1010 Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Blocking Peptide
1011 Cleaved Caspase-8 (Asp391) Blocking Peptide
1013 Jak1 Blocking Peptide
1015 EGR1 Blocking Peptide
1016 LEF1 Blocking Peptide
1021 Phospho-NF-kappaB p105 (Ser933) Blocking Peptide
1023 Phospho-IKK-alpha/beta (Ser176/180) Blocking Peptide
1025 β-Actin Blocking Peptide
1026 Phospho-Bad (Ser112) Blocking Peptide
1027 Phospho-GSK-3α (Ser21) Blocking Peptide
1032 β-Tubulin Blocking Peptide
1033 Cytochrome c Blocking Peptide
1034 COX IV Blocking Peptide
1035 Mre11 Blocking Peptide
1036 PU.1 Blocking Peptide
1037 Survivin Blocking Peptide
1038 Phospho-Stat1 (Tyr701) Blocking Peptide
1039 Jak2 Blocking Peptide
1044 Cyclin D1 Blocking Peptide
1047 Phospho-Ezrin (Thr567)/Radixin (Thr564)/ Moesin (Thr558) Blocking Peptide
1048 NEDD8 Blocking Peptide
1050 Cleaved Caspase-3 (Asp175) Blocking Peptide
1051 PP2A B Subunit Blocking Peptide
1052 Phospho-4E-BP1 (Thr37/46) Blocking Peptide
1053 4E-BP1 Blocking Peptide
1054 PP2A A Subunit Blocking Peptide
1055 p21 Waf1/Cip1 Blocking Peptide
1056 E-Cadherin Blocking Peptide
1057 Thioredoxin 1 Blocking Peptide
1059 HER2/ErbB2 Blocking Peptide
1061 NF-κB p65 Blocking Peptide
1062 Acetyl-CoA Carboxylase Blocking Peptide
1064 Tri-Methyl-Histone H3 (Lys4) Blocking Peptide
1066 TRAF1 Blocking Peptide
1067 PP2A C Subunit Blocking Peptide
1072 mTOR Blocking Peptide
1073 GSK-3β Blocking Peptide
1074 AMPKβ1/2 Blocking Peptide
1079 Stat1 Blocking Peptide (9175 Specific)
1081 DARPP-32 Blocking Peptide
1083 Acetyl-Histone H3 (Lys9) Blocking Peptide
1084 BiP Blocking Peptide
1085 Akt (pan) Blocking Peptide
1090 Phospho-CREB (Ser133) Blocking Peptide
1110 Phospho-EGF Receptor (Tyr1068) Blocking Peptide
1140 Phospho-Akt (Ser473) Blocking Peptide
1150 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Blocking Peptide
1155 S6 Ribosomal Protein Blocking Peptide
1160 Zap-70 Blocking Peptide
1170 Phospho-p38 MAPK (Thr180/Tyr182) Blocking Peptide
1175 Phospho-EGF Receptor (Tyr1173) Blocking Peptide
1180 Phospho-HER3/ErbB3 (Tyr1289) Blocking Peptide
1190 Phospho-cdc25C (Ser216) Blocking Peptide
1195 Phospho-Stat3 (Tyr705) Blocking Peptide
1205 p70 S6 Kinase Blocking Peptide
1210 Phospho-MEK1/2 (Ser221) Blocking Peptide
1220 Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide
1221 Phospho-eIF2α (Ser51) Blocking Peptide
1225 Bcl-xL Blocking Peptide
1230 Phospho-mTOR (Ser2448) Blocking Peptide
1235 Src Blocking Peptide
1240 p44/42 MAPK (Erk1/2) Blocking Peptide (#4695 Specific)
1245 p44/42 MAPK (Erk1/2) Blocking Peptide (#4696 Specific)
1250 PTEN Blocking Peptide
1254 Phospho-HER2/ErbB2 (Tyr1221/1222) Blocking Peptide
1255 Phospho-Chk2 (Thr68) Blocking Peptide
1260 Phospho-Histone H2A.X (Ser139) Blocking Peptide
1433 SRC-1 Blocking Peptide
1434 Di-Methyl-Histone H3 (Lys4) Blocking Peptide
1435 TrkA Blocking Peptide
1525 IGF-I Receptor β Blocking Peptide
1645 Phospho-Met (Tyr1234/1235) Blocking Peptide


< 中和抗体 >

中和抗体能够通过结合生物分子抑制其正常生物功能。这些试剂可以用来确定一个特定的大分子在生物系统中的影响。


No.  Name  Applications  Reactivity 
No.  Name  Applications  Reactivity 
11964 Human IL-4 Neutralizing (D20H1) Rabbit mAb  N  H 
11969 Mouse TNF-α Neutralizing (D2H4) Rabbit mAb  N  M 
12157 Human EGF Neutralizing (D8A1) Rabbit mAb  N  H 
12222 Mouse IL-3 Neutralizing (D6C1) Rabbit mAb  N  M 
12739 Human IL-17A Neutralizing (D13C2) Rabbit mAb  N  H 
48685 EGF Receptor (D1D4J) XP® Rabbit mAb (Neutralizing) (PE Conjugate)  F  H M 
54359 EGF Receptor (D1D4J) XP® Rabbit mAb (Neutralizing)  W IP IF-IC F N  H M 
7321 Human TNF-α Neutralizing (D1B4) Rabbit mAb  N  H 


应用：W=免疫印迹 IP=免疫沉淀 IHC-P=免疫组化(石蜡包埋) IHC-F=免疫组化 (冰冻)   IF-IC=免疫荧光 (免疫细胞化学) IF-F=免疫荧光 (冰冻)  F=流式细胞术 ChIP=染色质免疫沉淀

反应性：H=人  M=小鼠  R=大鼠  Hm=仓鼠 Mk=猴  C=鸡  Mi=貂  Dm=黑腹果蝇  X=非洲爪蟾 Z=斑马鱼  B=牛  Dg=狗 Pg=猪  Hr=马 

括号中的种属基于序列100%的同源性，预测可以反应。

请扫左方二维码进入，即可查看详细对照表格，并找CST授权代理商基因有限公司购买。


基因有限公司作为CellSignalingTechnology (CST) 优质一级代理商，为您实验提供更优的支持和帮助。想要更多相关知识和资料，或了解更多促销信息请在本文后留言或联系您身边的基因有限公司员工。



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