组织细胞破碎仪还能做这些？- 大数跨境

基因快讯

2024-02-29

导读：Bullet Blender组织细胞破碎仪可以代替研钵进行组织匀浆，帮助提取生物大分子。它是否还能有别的功能？让我们来一探究竟！

样品制备在生物学实验中看似简单，实则对实验结果影响很大。今天与大家一起分享一款在Cell、Nature、Science等著名期刊杂志中经常出现的组织细胞破碎仪——Bullet Blender Strom Pro。使用试管击打技术，转头以每分钟几千次的频率击打试管，使管内研磨珠与样品发生剧烈振动，从而彻底破碎样品。单次可处理1-24个样品；封闭式匀浆操作，避免样品交叉污染，轻松实现组织匀浆。

Bullet Blender Storm Pro具体有哪些应用？除了代替研钵进行组织匀浆，帮助提取生物大分子，它是否还能有别的功能？让我们来一探究竟！

不要小看它小小的身板，能支持的应用可不少。可从细菌、酵母、真菌、动植物组织、小型动物等样品中提取DNA、RNA、蛋白质、亚细胞结构或化学有机物质；还可对液体、半固体、固体样品进行快速匀浆；制备纳米颗粒或脂质体；从藻类植物中分离生物燃料等。

DNA提取

Bullet Blender可以较低的转速提取全长染色质，在处理过程中不会导致DNA发生过度剪切。

可提取完整的全长染色质

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Barr-Beare, E., Saxena, V., Hilt, E. E., Thomas-White, K., Schober, M., Li, B., … Schwaderer, A. L. (2015). The Interaction between Enterobacteriaceae and Calcium Oxalate Deposits. PLOS ONE, 10(10), e0139575.

Leach, M. D., Farrer, R. A., Tan, K., Miao, Z., Walker, L. A., Cuomo, C. A., … Cowen, L. E. (2016). Hsf1 and Hsp90 orchestrate temperature-dependent global transcriptional remodelling and chromatin architecture in Candida albicans. Nature Communications, 7, 11704.

Masterson, L., Winder, D. M., Ball, S. L. R., Vaughan, K., Lehmann, M., Scholtz, L.-U., … Goon, P. K. C. (2016). Molecular analyses of unselected head and neck cancer cases demonstrates that human papillomavirus transcriptional activity is positively associated with survival and prognosis. BMC Cancer, 16, 367.

RNA提取

Bullet Blender使用低能耗小功率电机，独特的空气冷却技术，保证样品破碎前后几乎无温差，避免温度敏感的生物大分子降解，得率高。

提取RNA分子完整，得率高

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Perland, E., Lekholm, E., Eriksson, M. M., Bagchi, S., Arapi, V., & Fredriksson, R. (2016). The Putative SLC Transporters Mfsd5 and Mfsd11 Are Abundantly Expressed in the Mouse Brain and Have a Potential Role in Energy Homeostasis. PLOS ONE, 11(6), e0156912.

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蛋白质提取

Bullet Blender可帮助提取组织或细胞中的蛋白质，用于下游蛋白质印迹、蛋白浓度或活性检测、或者蛋白质组学研究。

提取蛋白质未发生降解

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分离微生物&病毒

Bullet Blender 可用于从受感染的组织和植物材料中分离细菌和病毒。具体方案因样品而异，可参考官网推荐方案或参考文献操作。

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Erickson, A. K., & Pfeiffer, J. K. (2013). Dynamic Viral Dissemination in Mice Infected with Yellow Fever Virus Strain 17D. Journal of Virology, 87(22), 12392–12397.

Luethy, L. N., Erickson, A. K., Jesudhasan, P. R., Ikizler, M., Dermody, T. S., & Pfeiffer, J. K. (2016). Comparison of three neurotropic viruses reveals differences in viral dissemination to the central nervous system. Virology, 487, 1–10.

Kumar, M., Belcaid, M., & Nerurkar, V. R. (2016). Identification of host genes leading to West Nile virus encephalitis in mice brain using RNA-seq analysis. Scientific Reports, 6.

分离细胞器

一些细胞器，例如线粒体、叶绿体、细胞核和其他亚细胞结构，可以使用 Bullet Blender 从细胞中分离出来。为尽量减少对细胞器的损伤，需使用最温和的裂解条件。

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Drake, J. C., Bruns, D. R., Peelor, F. F., Biela, L. M., Miller, R. A., Hamilton, K. L., & Miller, B. F. (2014). Long-lived crowded-litter mice have an age-dependent increase in protein synthesis to DNA synthesis ratio and mTORC1 substrate phosphorylation. AJP: Endocrinology and Metabolism, 307(9), E813–E821.

Al-Hasan, Y. M., Evans, L. C., Pinkas, G. A., Dabkowski, E. R., Stanley, W. C., & Thompson, L. P. (2013). Chronic Hypoxia Impairs Cytochrome Oxidase Activity Via Oxidative Stress in Selected Fetal Guinea Pig Organs. Reproductive Sciences, 20(3), 299–307.

制备纳米颗粒/脂质体

Bullet Blender 可帮助制备尺寸一致的纳米级颗粒或脂质体。可以通过增加均质化时间来减小纳米颗粒的粒度。

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Yang, X., Wang, D., Ma, Y., Zhao, Q., Fallon, J. K., Liu, D., … Liu, F. (2014). Theranostic nanoemulsions: codelivery of hydrophobic drug and hydrophilic imaging probe for cancer therapy and imaging. Nanomedicine, 9(18), 2773–2785.

Kim, K.-T., Zaikova, T., Hutchison, J. E., & Tanguay, R. L. (2013). Gold Nanoparticles Disrupt Zebrafish Eye Development and Pigmentation. Toxicological Sciences, 133(2), 275–288.

Fu, Q., Wang, Y., Ma, Y., Zhang, D., Fallon, J. K., Yang, X., … Liu, F. (2015). Programmed Hydrolysis in Designing Paclitaxel Prodrug for Nanocarrier Assembly. Scientific Reports, 5, 12023.