【细胞小讲堂】DU-145全面剖析：特点、应用、培养方法与研究进展

在使用细胞系之前，了解细胞系的来源和一般特征至关重要。

• DU-145是一种人类前列腺癌细胞系，来源于一名患有转移性前列腺癌的高加索男子（69岁）的大脑。

• 与PC-3前列腺癌细胞一样，DU-145也表达雄激素受体。然而，当这些细胞接受雄激素配体处理时，它们不显示任何活性的雄激素受体响应基因。因此，DU145被认为是雄激素非依赖性细胞。

• DU-145细胞呈上皮形态。

• DU-145细胞是低三倍体，模态染色体数为64。它们表现出几条标记染色体，例如del（11）（q23），t（11q12q），16q+，del（1）（p32）和del（9）（p11）。

PC-3与DU145两种前列腺癌细胞系都是雄激素受体非依赖性的。它们在致瘤和转移潜力方面有所不同。当注射到免疫功能低下的小鼠中时，PC-3细胞系形成高度转移的IV级腺癌。DU-145细胞系则发展为具有中度转移潜力的前列腺癌[1]。

2.培养条件

细胞来源：前列腺;转移灶

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：EMEM (Eagle's Minimum Essential Medium) with medium supplements, i.e., 10% Fetal Bovine Serum (FBS), 2 mM L-Glutamine, 2.2 g/L NaHCO3, and EBSS.

推荐换液：2~3次/周

传代比例：1:3-1:4

倍增时间：~28-51 hours

保藏机构：ATCC;HTB-81;CCTCC;GDC0704

培养条件：气相：空气，95%；CO2，5%；温度：37℃

冻存条件：基础培养基+5%DMSO+40%FBS，液氮保存

生物安全等级：BSL1

01.

优点

02.

缺点

前列腺癌生物学：DU-145是研究前列腺癌生物学的相关模型。研究人员利用这些细胞来研究驱动癌症发展、进展和转移的分子机制。此外，还探索了与前列腺癌相关的遗传改变。2021 年进行的研究检查了 DU-145 细胞，以确定潜在的生物标志物，从而具有有效的诊断和治疗意义。该研究表明肌动蛋白γ1（ACTG1）是一种潜在的前列腺癌生物标志物，因为它通过MAPK / ERK途径激活促进癌细胞增殖并调节转移[2]。

药物筛选和开发：DU-145前列腺癌细胞被广泛用于测试潜在抗癌药物的细胞毒性和功效。这有助于研究人员识别新药，了解耐药性和潜在的分子机制。2022 年进行的一项研究探讨了树脂混合物、蜂胶或蜂胶在前列腺癌细胞系 PC-3 和 DU-145 中的细胞毒性作用。该研究显示了这种天然物质在两种细胞系中的生长抑制作用，并提出了其抗癌潜力[3]。另一项研究于 2019 年进行，调查了天然化合物甜菜碱在 DU-145 前列腺癌细胞中的抗增殖作用。研究表明，甜菜碱通过提高DU-145细胞中氧化应激介导的细胞死亡和炎症来发挥抗增殖活性[4]。

The following are some research publications on the DU-145 cell line.

Concentration-dependent effects of zinc sulfate on DU-145 human prostate cancer cell line: oxidative, apoptotic, inflammatory, and morphological analyzes

https://idp.springer.com/authorize/casa?redirect_uri=https://link.springer.com/article/10.1007/s12011-019-01879-0&casa_token=ZRF6BXTOmQsAAAAA:ZEruNaRe8ioBWzjk54bCtJi3bFNdXirkxhCdMLoKjaJKY5gl5ajbWJzpx0rWBUjwAtigqEmt5CqqnnoA

This study in the Biological Trace Element Research, 2020, proposed that zinc sulfate (ZnSo(4)) exerts anti-proliferative effects in DU-145 cells via inducing apoptosis, morphological changes, oxidative damage, and inflammation.

Selective cytotoxic and anti-metastatic activity in DU-145 prostate cancer cells induced by Annona muricata L. bark extract and phytochemical, annonacin

https://link.springer.com/article/10.1186/s12906-020-03130-z

This research article was published in BMC Complementary Medicine and Therapies in 2020. This study explored the cytotoxic and anti-metastatic potential of a plant, Annona muricata L. bark extract, and its bioactive annonacin in the DU-145 cell line.

LncRNA LOXL1‐AS1/miR‐let‐7a‐5p/EGFR‐related pathway regulates the doxorubicin resistance of prostate cancer DU‐145 cells

https://iubmb.onlinelibrary.wiley.com/doi/abs/10.1002/iub.2075

This publication in IUBMB Life (2019) proposed that long non-coding RNA LOXL1-AS1 interaction with miR-let-7a-5p and EGFR pathway regulate the doxorubicin resistance in DU-145 prostate cancer cells.

Down-regulation of DDR1 induces apoptosis and inhibits EMT through phosphorylation of Pyk2/MKK7 in DU-145 and Lncap-FGC prostate cancer cell lines

https://www.ingentaconnect.com/content/ben/acamc/2020/00000020/00000008/art00012

This article in Anti-Cancer Agents in Medicinal Chemistry (2020) stated that Discoidin Domain Receptor1 (DDR1) gene downregulation causes DU-145 cells death and inhibits epithelial to mesenchymal transition (EMT) via activating Pyk2/MKK7 pathway.

Casticin inhibits human prostate cancer DU 145 cell migration and invasion via Ras/Akt/NF‐κB signaling pathways

https://onlinelibrary.wiley.com/doi/abs/10.1111/jfbc.12902?casa_token=EFaTNq1pHK8AAAAA:8TpD5WVyy2FeRYqPDdxK3eakK0W1O1Y9I16mlR7Ev789p2nbwLuOOQyAtbFCq6Ehs3MJ7NUPdSR_cEs

This study in the Journal of Food Biochemistry (2019) proposed that Casticin, a polymethoxyflavone, inhibits migration and invasion of DU-145 cells by regulating Ras/Akt/NF‐κB signalling.

Many online resources are available on the DU-145 prostate cancer cell line. Here this section will mention a few resources explaining the handling, maintenance, and transfection protocol for these cells:

• DU-145 transfection: This video tutorial is a step-wise guide for learning the protocol for transfecting DU-145 cells.https://www.youtube.com/watch?v=wILkmtv4eIg

The DU-145 cell culture protocol is mentioned here.

• DU-145 subculturing: This link will help you learn the protocol for subculturing or sub-cultivating DU-145 cells.https://bio-protocol.org/exchange/minidetail?type=30&id=9081983

• DU-145 cells: This website contains plenty of useful information about the DU-145 cell line, including DU145 medium, protocol for subculturing, and handling proliferative and cryopreserved cultures.

https://cytion.com/DU-145/300168

因篇幅原因，欲了解更多DU145 详细信息，如：STR profile、Publications、Hierarchy、HLA typing、Cell line collections 等等。

请点击链接查看https://wiki.taizecell.cn/index.php?title=DU145

或扫码输入DU145进行查询

参考文献

1. Lima, A.R., et al., Discrimination between the human prostate normal and cancer cell exometabolome by GC-MS. Scientific reports, 2018. 8(1): p. 5539.

2. Xiao, L., et al., Silencing ACTG1 expression induces prostate cancer epithelial mesenchymal transition through MAPK/ERK signaling pathway. DNA and Cell Biology, 2021. 40(11): p. 1445-1455.

3. Gogacz, M., et al., Anticancer effects of propolis extracts obtained with the cold separation method on PC-3 and DU-145 prostate cancer cell lines. Molecules, 2022. 27(23): p. 8245.

4. Kar, F., et al., Betaine suppresses cell proliferation by increasing oxidative stress–mediated apoptosis and inflammation in DU-145 human prostate cancer cell line. Cell Stress and Chaperones, 2019. 24: p. 871-881.