流式“六月”
BD与赛哲一起为流式奉献！
四大促销，等你来选
1、一次性购买BD产品大于6400元，即可获得一份双人电影劵；
2、BD热卖磁珠产品，买十送一（或等价流式抗体）；
3、556547、559763 BD凋亡试剂盒4.2折，劲爆促销；
4、单笔订单满两支Sirigen新染料（BB、BV、BUV、APC-R700），整单BD流式抗体享5折优惠。
556547（FITC-Annexin V/PI），实验步骤及结果：
1、Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of 1X106 cells/ml
2、Transfer 100ul of the solution to a 5ml culture tube
3、Add 5ul of FITC Annexin V and 5ul PI
4、Gently vortex the cells and incubate for 15min at RT in the dark
5、Add 400ul of 1X Binding Buffer to each tube. Analyze by flow cytometry within 1hr.
IMag介绍
BDTM IMag磁珠直径约200nm，综合了大磁珠和小磁珠的优点，简便、快捷和有效地分选特定细胞，保证了纯度和得率，而且不会对细胞造成损伤。
BD Imag磁珠 |
大磁珠 |
小磁珠 |
|
磁珠大小 |
100~450nm |
1200~4500nm |
≈50nm |
分离得率 |
纯度＞95%，得率＞95% |
纯度＞95%，得率＞90% |
|
分离过程对细胞的影响 |
对细胞温和，分离后无需去除磁珠，即可用于分析和后续实验 |
对细胞造成机械压力，影响其生物学功能和活性，不利于后续的分析和培养 |
必须使用较强的磁场，增加磁分离系统的成本 |
分离速度 |
速度快，得率高，成本低 |
速度慢，纯度高但得率较低 |
|
应用成本 |
技术简单，可在普通流式管中完成分离 |
技术简单，可在普通试管中完成分离 |
不能在普通试管中进行，每次试验需更换一次性分离柱，成本昂贵 |
对后续流式实验的影响 |
上流式时不影响爱那个散射光，易于增减细胞用量 |
容易阻塞FCM喷嘴 |
上流式时不影响散射光 |
IMag“买十送一”清单
货号 |
名称 |
货号 |
名称 |
551513 |
Ms CD45R/B220 IMag-DM RA3-6B2 10mL |
557983 |
Ms IgG1 IMag Particles-DM A85-1 5mL |
551516 |
Ms CD8a IMag Particles-DM 53-6.7 10mL |
557986 |
Hu CD45RO IMag Particles-DM UCHL1 5mL |
551518 |
Ms CD90.2 Thy1.2 IMag-DM 30-H12 10mL |
557987 |
Hu NK Cell Enrichment Set IMag-DM |
551520 |
Hu CD19 IMag Particles-DM HIB19 5mL |
558002 |
Rat Ig Kpa Light Chain IMag-DM MRK-1 |
551539 |
Ms CD4 IMag Particles-DM GK1.5 10mL |
558004 |
Ms NK Cell Separation Set IMag-DM |
552311 |
IMag Cell Separation Magnet |
558005 |
Hu CD25 IMag Particles-DM 2A3 5mL |
552362 |
IMag Buffer 10X 100mL |
558013 |
Ms CD11b IMag-DM M1/70 10mL |
552593 |
Hu CD3 IMag Particles-DM HIT3a 5mL |
558111 |
Ms Ly-6G/Ly-6C IMag-DM RB6-8C5 10mL |
557766 |
Hu CD8 IMag Particles-DM SK1 5mL |
558131 |
Ms CD4 T LYM Enrichment Set IMag-DM |
557767 |
Hu CD4 IMag Particles-DM L200 5mL |
558420 |
Hu Dndrtc Cell Enrch Set IMag- DM |
557769 |
Hu CD14 IMag Particles-DM MfP9 5mL |
558451 |
Ms Stem Cell Enrichment Set IMag - DM |
557775 |
Hu CD56 IMag Particles-DM NCAM16.2 5mL |
558454 |
Hu Monocyte Enrichment Set IMag- DM |
557792 |
Ms B Lym Enrichment IMag-DM Set |
558471 |
Ms CD8 T Lympho Enrch IMag-DM Set |
557793 |
Ms T Lym Enrichment IMag-DM Set |
558497 |
Hu CD19 IMag-DM 4G7 5mL |
557812 |
Strpv IMag Particles Plus-DM 5mL |
558520 |
Hu Memory CD4 T Enrch Set IMag-DM |
557874 |
Hu T Lym Enrichment IMag-DM Set |
558521 |
Hu Naive CD4 T Cell Enrch Set IMag-DM |
557899 |
R-PE IMag Particles-DM E31-1459 5mL |
558536 |
Ms Ter-119 IMag-DM TER-119 5mL |
557932 |
APC IMag Particles-DM E30-221 5mL |
558569 |
Hu Naive CD8 T Cell Enrch Set IMag-DM |
557939 |
Hu CD4 T Lym Enrichment IMag-DM Set |
558620 |
Ms CD117 Magnetic Particles-DM 2B8 5mL |
557941 |
Hu CD8 T Lym Enrichment IMag-DM Set |
558662 |
Cell Cycle Bioimaging Kit 100 Tests |
557954 |
Ms NK Cell Enrichment Set IMag-DM |
558705 |
Hu CD11c IMag-DM S-HCL-3 5mL |
557981 |
Hu CD45RA IMag Particles-DM HI100 5mL |
560030 |
Hu Lineage Cell Depletion IMag-DM Set |
广州赛哲科技有限公司
地址：广州国际生物岛标准产业单元二期螺旋四路9号4栋C区301
电话：4008-631-632
网址：www.sagenetech.com
