厌氧菌革兰氏阴性菌以及其他的众多病原菌在进行在进行生理活动的时候，都会有特异性的有机酸或者脂肪酸的代谢或者分泌，利用这些特点，可以轻松的对特殊的致病菌进行识别和鉴定。同时还可以检测病原菌的活跃程度以及判断不同的病理过程。Aminex 在有机酸分析以及脂肪酸鉴定方面的优势在病原菌鉴定领域有着独特的应用。
更多的信息请大家参考下表文献！
以下是 Aminex 色谱柱在病原菌检测领域的应用文献汇总。
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1. 高效液相色谱法和气相色谱法测定嗜碱细菌培养基中有机酸的比较 |
Paavilainen, S. and Korpela, T., J. Chrom., 634, 273-280 (1993). Comparison of High-Performance Liquid and Gas Chromatography in the Determination of Organic Acids in Culture Media of Alkaliphilic Bacteria. |
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2. 分析从病原细菌中提取的胞外多糖 |
Radin, L., Sivori, S., Eftimiadi, C. and Lucarelli, C., Biomed. Chrom., 6, 115-119 (1992).HPLC Strategy Suitable for the Analysis of Exopolysaccharides Extracted from Pathogenic Bacteria. |
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3. 鉴定厌氧细菌的改进程序 - 短链脂肪酸的定量分析 |
Krausse R. and Ullmann, U., Zbl. Bakt. Hyg., A, 276, 1-8 (1991). A Modified Procedure for the Identification of Anaerobic Bacteria by HPLC - Quantitative Analysis of Short-Chain Fatty Acids. |
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4. HPLC 在革兰氏阴性细菌细胞壁和脂多糖成分的鉴定和定量中的应用 |
Williams, J. W., Saravolac, E. G. and Taylor, N. F., Microchem. J., 43, 2-9(1991).The Use of HPLC in the Identification and Quantification of Cell Wall and Lipopolysaccharide Components in Gram Negative Bacteria. |
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5. 通过 HPLC 快速鉴定拟杆菌属 |
Radin, L., Arzese, A., Lucarelli, C. and Botta, G. A., J. Chrom., 459, 331-335 (1988).Rapid Identification of Bacteroides Species by HPLC. |
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6. 使用光电二极管阵列检测对尿液羧酸进行液相色谱分析来早期识别异戊酸尿症 |
Krausse, R. and Ullmann, U., Zbl. Bakt. Hyg., A, 265, 340-352 (1987). Identification of Anaerobic Bacteria Using HPLC. 108. Buchanan, D. N. and Thoene, J. G., Biomed. Chrom., 1, 38-40 (1986). Early Identification of Isovaleric Aciduria Using Photodiode Array Detection for Liquid Chromatographic Profiling of Urinary Carboxylic Acids. |
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7. 使用生化测试和高效液相色谱法鉴定弯曲杆菌物种的研究 |
Krausse, R. and Ullmann, U., Zbl. Bakt. Hyg., A, 260, 342-360 (1985). Studies on the Identification of Campylobacter Species Using Biochemical Tests and HPLC. |
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8. 微生物酸代谢物的高效液相色谱 |
Adams, R. F., Jones, R. L. and Conway, P. L., J. Chrom., 336, 125-137 (1984).High-Performance Liquid Chromatography of Microbial Acid Metabolites. |
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9. 气液与 HPLC 和质谱的比较，用于巴西固氮螺菌和脱硫弧菌培养基中有机酸的检测 |
Loh, W. H.-T., Santoro, N., Miller, R. H. and Tuovinen, O. H., J. Gen.Microbiol., 130, 1051-1059 (1984).Comparison of Gas-Liquid and HPLC, and Mass Spectrometry, for the Detection of Organic Acids in Culture Media of Azospirillum brasilense and Desulfovibrio desulfuricans. |
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10. 通过 HPLC 分析厌氧细菌的短链酸 |
Guerrant, G. O., Lambert, M. A. and Moss, C. W., J. Clin. Microbiol., 16,355-360 (1982). Analysis of Short-Chain Acids from Anaerobic Bacteria by HPLC. |
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11. 厌氧细菌鉴定中发酵产物分析的液相色谱程序 |
Ehrlich, G. G., Goerlitz, D. F., Bourell, J. H., Eisen, G. V. and Godsy, E. M.,Appl. Environ. Microbiol., 42, 878-885 (1981). LC Procedure for Fermentation Product Analysis in the Identification of Anaerobic Bacteria. |
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