BMG多功能酶标仪国内用户出版物快报 (2025年10月)

>>>Piezo1 activation suppresses bone marrow adipogenesis to prevent osteoporosis by inhibiting a mechanoinflammatory autocrine loop

香港大学药物生物技术国家重点实验室，香港大学医学系，香港大学粤港代谢医学联合实验室研究团队近日在高影响因子专业学术期刊 signal transduction and targeted therapy上发表题为 Piezo1 activation suppresses bone marrow adipogenesis to prevent osteoporosis by inhibiting a mechanoinflammatory autocrine loop 的研究文章，影响因子高达52！研究发现来源于多能骨髓间充质干细胞的骨髓脂肪细胞（BMMSC）中的机械敏感阳离子通道Piezo1是骨髓脂肪生成的关键抑制因子。BMMSCs 中的 Piezo1 激活通过阻止 Ccl2-Lcn2 炎症自分泌环来抑制骨髓脂肪生成以维持骨强度，从而揭示了机械转导、炎症和细胞命运决定之间以前未被认识到的联系。 研究人员通过荧光素酶双报告基因测定Lcn2或Ccl2启动子强度，并使用具有双荧光素酶检测功能的 CLARIOstar 酶标仪（BMG LABTECH）定量荧光素酶活性。萤火虫荧光素酶活性值根据海肾荧光素酶读数进行归一化，以解释转染效率的变化，相对启动子活性表示为萤火虫与海肾发光信号的比率。                  

BMG的CLARIOstar 酶标仪具有多重荧光素酶检测能力，内置的注射系统可自动添加底物和终止液，全自动进行双荧光素酶检测。针对荧光信号变化，CLARIOstar 酶标仪动态范围扩展技术可免去实验条件的优化步骤，动态范围单次检测可达8个数量级，不用担心添加底物之后信号增加后的溢出风险，为实验室平台和广大科研人员提供最佳的检测数据。

（原文：https://www.nature.com/articles/s41392-025-02455-w）

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Piezo1 activation suppresses bone marrow adipogenesis to prevent osteoporosis by inhibiting a mechanoinflammatory autocrine loop

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