BMG多功能酶标仪国内用户出版物快报 (2025年1月)

>>>Programmable trans-splicing riboregulators for complex cellular logic computation

浙江大学化学与生物工程学院，浙江大学-杭州全球科技创新中心王宝俊团队在高影响因子专业学术期刊Nature Chemical Biology上发表题为Programmable trans-splicing riboregulators for complex cellular logic computation的研究文章，研究团队开发了一类基于断裂内含子反式剪接的基因调控技术（SENTRs），SENTR库提供低泄漏表达、宽动态范围、高机器学习可预测性以及多组分水平的低串扰。SENTR技术具有优越的可编程性、可预测性、正交性、灵活性，可用于RNA检测、RNA逻辑计算以及断裂生物分子的多输入复杂逻辑计算。正交性是衡量基因元件质量的一项重要指标，它指多个元件同时使用时，相互之间互不干扰的能力。在正交 SENTR 的设计和评估过程中， 研究团队采用BMG的FLUOstar Omega读取荧光，计算荧光比值获得串扰水平。研究团队也对RNA反式剪接系统的灵活性、稳健性以及应用进行了进一步的探索。通过下游报告基因检测，评估其调控功能。研究团队采用BMG的FLUOstar Omega分别读取绿色荧光、红色荧光、蓝色荧光和OD600, 使用Omega MARS 3.32 software数据分析软件进行荧光/OD600标准化处理，以检测报告基因。                           

德国BMG的FLUOstar Omega多功能酶标仪的能进行同时进行多色荧光和OD600吸光度检测，标配的分析软件可轻松计算荧光/OD600的比值进行标准化处理，不仅仅在生物合成领域，在基因组学，神经退行性疾病诊断，微生物生长监测等领域都已经得到广泛的应用和用户认可。

（原文：https://www.nature.com/articles/s41589-024-01781-4）

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