BMG多功能酶标仪国内用户出版物快报 (2023年4月)

>>>Engineering of NEMO as calcium indicators with large dynamics and high sensitivity

中国科学技术大学生命科学与医学部合肥国家微观物理科学研究中心，中国科学院脑功能与疾病重点实验室，合肥国家综合科学中心人工智能研究所，北京师范大学认知神经科学与学习国家重点实验室，IDG/麦戈文脑研究所，北京师范大学生命科学学院基因资源与分子发育北京市重点实验室，北京师范大学生命科学学院细胞增殖与调控生物学教育部重点实验室近日联合在高分专业学术期刊Nature Methods发表了学术研究成果：Engineering of NEMO as calcium indicators with large dynamics and high sensitivity，报道了一套钙的传感器NEMO，具有快速动力学和宽动态范围（>100倍）。NEMO指标报告Ca2+瞬变，可以检测到SBR峰值神经元的单一动作电位体内SBR的中位峰值是体内SBR的两倍和四倍，优于大多数现有最先进的钙指示剂。新的钙指示剂报告细胞内Ca2+信号具有高度的灵敏度和分辨率，在监测神经元活动，哺乳动物和植物体内Ca2+调节的生理过程等方面能得到广泛应用。研究人员选用了德国的BMG Labtech POLARstar Omega多功能酶标仪来进行钙指示剂解离常数的检测。BMG Labtech POLARstar Omega多功能酶标仪配备2个在位注射器，能在加试剂的同时完成检测，无任何延迟，特别适用于钙瞬变的实时检测。

（原文：https://www.nature.com/articles/s41592-023-01852-9）

Trehalose metabolism targeting as a novel strategy to modulate acid tolerance of yeasts and its application in food industry

江南大学谷物发酵与食品生物制造国家工程研究中心

Berberine activates PPARδ and promotes gut microbiota-derived butyric acid to suppress hepatocellular carcinoma

香港中文大学中药研究所

Platelet Membrane Fragment Self-Assembled Oral Hydrogel Microspheres for Restoring Intestinal Microvascular Injury

上海交通大学医学院附属瑞金医院上海市创伤骨科研究所

Calycosin alleviates cerebral ischemia/reperfusion injury by repressing autophagy via STAT3/FOXO3a signaling pathway

浙江中医药大学药学院

Identification of Binding Sites in the Nucleotide-Binding Domain of P-Glycoprotein for a Potent and Nontoxic Modulator, the Amine-Containing Monomeric Flavonoid FM04

香港理工大学应用生物与化学技术系

Nanodroplet-based reagent delivery into water-in-fluorinated-oil droplets

中国环境科学研究院环境健康风险评价与研究中心

FURIN suppresses the progression of atherosclerosis by promoting macrophage autophagy

青岛大学医学院

Bioactive compounds from Huashi Baidu decoction possess both antiviral and anti-inflammatory effects against COVID-19

中国科学院微生物研究所中国科学院病原微生物学与免疫学重点实验室

ZBTB16 eases lipopolysaccharide‑elicited inflammation, apoptosis and degradation of extracellular matrix in chondrocytes during osteoarthritis by suppressing GRK2 transcription

广西中医药大学第一附属医院骨科创伤科

Exosomes loading Tapasin enhance T cell immune response by autophagy to inhibit HBV replication

湖北工业大学

Engineering of NEMO as calcium indicators with large dynamics and high sensitivity

中国科学技术大学生命科学与医学部合肥国家微观物理科学研究中心

Dynamics of soil net nitrogen mineralization and controlled effect of microbial functional genes in the restoration of cold temperate forests

华北电力大学工程生态与非线性科学研究中心

Dynamics of soil net nitrogen mineralization and controlled effect of microbial functional genes in the restoration of cold temperate forests

华北电力大学工程生态与非线性科学研究中心

Histone chaperone SSRP1 is required for apoptosis inhibition and mitochondrial function in HCC via transcriptional promotion of TRAP1

吉林大学整形外科

Role of lncRNA MAGI2-AS3 in lipopolysaccharide-induced nucleus pulposus cells injury by regulating miR-374b-5p/interleukin-10 axis

武汉江汉大学附属医院骨科

Chitooligosaccharide-catechin conjugate: Antimicrobial mechanisms toward Vibrio parahaemolyticus and its use in shucked Asian green mussel

浙江海洋大学食品科学与药学院浙江省海产品健康危险因素重点实验室

EGFR inhibitor erlotinib plus monoclonal antibody versus erlotinib alone for first-line treatment of advanced non-small cell lung carcinoma: A systematic review and meta-analysis

四川大学华西医院生物治疗与肿瘤中心国家重点实验室

Metabolic reprogramming driven by IGF2BP3 promotes acquired resistance to EGFR inhibitors in non-small cell lung cancer

中山大学

Menstrual blood-derived endometrial stem cells alleviate neuroinflammation by modulating M1/M2 polarization in cell and rat Parkinson's disease models

新乡医学院医学工程学院河南干细胞医学国际联合研究实验室

Ozone-oxidized black carbon particles change macrophage fate: Crosstalk between necroptosis and macrophage extracellular traps

北京大学公共卫生学院毒理学教研室

Rare species contribute greater to ecosystem multifunctionality in a subtropical forest than common species due to their functional diversity

中国林业科学研究院高原森林科学研究所

Maternal zinc alleviates tert-butyl hydroperoxide-induced mitochondrial oxidative stress on embryonic development involving the activation of Nrf2/PGC-1α pathway

华南农业大学畜禽育种国家重点实验室

Fusobacterium nucleatum aggravates rheumatoid arthritis through FadA-containing outer membrane vesicles

南方医科大学南方医院风湿病中医中心

SARS-CoV-2 infection aggravates cigarette smoke-exposed cell damage in primary human airway epithelia

香港大学李嘉诚医学院临床医学院医学系

Berberine Enhances Intestinal Mucosal Barrier Function by Promoting Vitamin D Receptor Activity

广州中医药大学附属第一医院消化内科

Structural insights into angiotensin receptor signaling modulation by balanced and biased agonists

浙江大学药学院

BIND&MODIFY: a long-range method for single-molecule mapping of chromatin modifications in eukaryotes

华大基因

Online detection of alkanes by a biological-phase microextraction and biosensing (BPME-BS) device

吉林大学地下水资源与环境教育部重点实验室

Structures of L-proline trans-hydroxylase reveal the catalytic specificity and provide deeper insight into AKG-dependent hydroxylation

中国科学技术大学生命科学与医学系

Optimization of nonspecific protease activity fabrication by Bacillus subtilis N30 isolated from Taiwan using different models of response surface methodology

国立屏东科技大学食品科学系

Nasopharyngeal carcinoma cells promote regulatory T cell development and suppressive activity via CD70-CD27 interaction

香港大学李嘉诚医学院临床肿瘤学系

Screening and Optimization of Fermentation Medium for Bacillus velezensis BP-1 and Its Biocontrol Effects against Peyronellaea arachidicola

辽宁省农业科学院植物保护研究所

Lateral flow immunoassay for rapid and sensitive detection of dsRNA contaminants in in vitro-transcribed mRNA products

中国科学院国家纳米科学技术中心

The Synergistic Antimicrobial Effect and Mechanism of Nisin and Oxacillin against Methicillin-Resistant Staphylococcus aureus

西安交通大学公共卫生学院

Bub1 and Bub3 regulate metabolic adaptation via macrolipophagy in Drosophila

中山大学生命科学学院

The composition, function and assembly mechanism of epiphytic microbial communities on Gracilariopsis lemaneiformis

暨南大学水生生物研究所

QTL mapping reveals both all-stage and adult-plant resistance to powdery mildew in Chinese elite wheat cultivars

中国农业科学院作物科学研究所

Promoted Skin Wound Healing by Tail-Amputated Eisenia foetida Proteins via the Ras/Raf/MEK/ERK Signaling Pathway

北京中医药大学中药学院

Targeting a novel circITCH/miR-421/BTG1 axis is effective to suppress the malignant phenotypes in hepatocellular carcinoma (HCC) cells

哈尔滨医科大学第四附属医院普外科

Phyllanthus emblica L. extract alleviates leptin resistance and lipid accumulation by inhibiting methylglyoxal production

国立中兴大学食品科学与生物技术系

Methyl vanillate for inhibiting the proliferation, migration, and epithelial-mesenchymal transition of ovarian cancer cells via the ZEB2/Snail signaling pathway

四川大学华西医院深部地下空间医学中心