BMG多功能酶标仪国内用户出版物快报 (2025年9月)

>>>Calhm6 Governs Macrophage Polarization Through Chp1-Camk4-Creb1 Axis and Ectosomal Delivery in Inflammatory Responses

山东第一医科大学山东省肿瘤医院研究所，山东省精准肿瘤重点实验室，齐鲁医院胃肠外科研究团队近日在高影响因子专业学术期刊International Journal of Molecular Medicine上发表题为Calhm6 Governs Macrophage Polarization Through Chp1-Camk4-Creb1 Axis and Ectosomal Delivery in Inflammatory Responses的研究文章，研究CENPU 表达水平与肿瘤之间的关联。功能分析表明，CENPU促进干细胞样行为和致瘤性，并诱导BC细胞恶性肿瘤。从机制上讲，CENPU通过抑制其溶酶体降解来促进弗林蛋白酶活性。CENPU 促进肿瘤发生，弗林蛋白抑制剂治疗抑制了这种作用。本研究的结果表明，CENPU 在导致肿瘤发生的弗林蛋白酶介导的信号传导中起着关键作用。因此，CENPU可能是TNBC中的一种新分子靶点。

弗林蛋白活性通过评估细胞消化底物的能力来测量，每 5 分钟进行一次测量，总持续时间为100分钟。使用荧光酶标仪动态检测弗林蛋白的催化活性。Km 值通过拟合酶活性数据的 Michaelis-Menten 曲线（速度与底物浓度）。Km 是在反应速度达到 Vmax 一半的底物浓度。       

酶促动力学采用了BMG的FLUOstar OPTIMA荧光酶标仪，FLUOstar OPTIMA型号稳定耐用，使用多年依然拥有出色的检测性能。BMG自带的分析功能配置米氏常数分析功能，为用户进行酶促动力学曲线分析工具。

（原文：https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.202502395）

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Calhm6 Governs Macrophage Polarization Through Chp1-Camk4-Creb1 Axis and Ectosomal Delivery in Inflammatory Responses

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