芯片VS质谱
Array vs MS
抗体芯片和质谱都是目前做蛋白质组学研究中常用的两种工具。
但是在技术原理上却有着天差地别的区别，所以在应用中也各有优劣势。
芯片：基于抗原抗体结合，在检测灵敏度和准确性更高。
质谱：基于分子质荷比计算及数据库肽段结构和完整性的比对，可以在许多种属样本中发现新的物质。
帮助基础研究及临床科研工作者从最完美的蛋白检测服务中找到最需要的蛋白组信息！
RayBio精准蛋白质组学检测服务参考方案
方案1：高精准低丰度蛋白检测方案
质谱（DIA）+抗体芯片（例如GSM-CAA-4000,检测200种低表达核心功能细胞因子）
方案2：超广谱蛋白检测方案
质谱（Label free）+抗体芯片（例如GSM-CAA-4000）
方案3：单独质谱高通量蛋白检测
Label free/DIA
方案4：单独芯片高通量蛋白检测
AAH/M/R-BLG（1000-8000）
（检测人/小鼠/大鼠中1000-8000个蛋白质），检测指标远超现在质谱最高检出指标
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更多检测技术搭配方案请咨询我们！
抗体芯片&MS联用的优势
检测结果1+1>2
非靶向MS的无偏检测&抗体芯片的高灵敏度相结合，它们互补将带来的前所未有的研究潜力，可以获得更大的蛋白质组覆盖率和更多的生物学见解。
检测价格1+1<2
芯片独家价格优势+如今市面上质谱价格早已被打下来，简直花小钱办大事。
Array&MS联用研究技术路线1
适用于生物标志物筛选、药物作用机制及成果转化研究
1.
实验组&对照组利用MS进行蛋白广筛
获得大量差异蛋白信息
2.
差异蛋白GO/KEGG分析
筛选相关分子功能或通路
3.
根据筛选出来的通路选用合适的抗体芯片验证，获得更确切的差异信息
相关芯片推荐
4.
结合队列研究例如数字模型构建确认主要靶点信息开发临床试剂盒
Array&MS联用研究技术路线2
疾病机理研究、人群分流、蛋白信息库建立等适用
1.
实验组&对照组利用MS及抗体芯片进行蛋白广筛
获得大量差异蛋白信息
2.
绘制蛋白表达图谱观察不同处理整体蛋白表达的异同
3.
差异蛋白GO/KEGG分析筛选相关通路及功能
Array&MS联用研究技术路线3
磷酸化通路检测、疾病机理研究、药物药效研究等
1.
利用MS筛选疾病组及健康组磷酸化蛋白差异
2.
利用磷酸化抗体芯片验证及筛选主要差异蛋白
获得确切靶点氨基酸磷酸化修饰信息
3.
建立疾病相关细胞或生物模型，利用磷酸化ELISA筛选最佳作用药物或剂量
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其他更多研究方案推荐及技术搭配请联系我们！！
近期优惠活动
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▼ RayBio®抗体芯片近期部分学术引用
| Year | Journal | 2023 IF | Article Title |
| 2017 | Nature Communications | 16.6 | Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis |
| 2017 | Nature Immunology | 30.5 | Direct control of regulatory T cells by keratinocytes |
| 2017 | Nature | 64.8 | Cytoplasmic chromatin triggers inflammation in senescence and cancer |
| 2017 | Nature Medicines | 82.9 | Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms |
| 2018 | Acta Biomaterialia | 9.7 | Manipulation of the response of human endothelial colony-forming cells by focal adhesion assembly using gradient nanopattern plates |
| 2018 | Journal for Immuno Therapy of Cancer | 10.9 | Randomized phase II trial of autologous dendritic cell vaccines versus autologous tumor cell vaccines in metastatic melanoma 5-year follow up and additional analyses |
| 2018 | Arthritis & Rheumatology | 13.3 | Myogenic Progenitor Cells Exhibit Type I Interferon–Driven Proangiogenic Properties and Molecular Signature During Juvenile Dermatomyositis |
| 2018 | Hepatology | 13.5 | Identification and Therapeutic Intervention of Coactivated Anaplastic Lymphoma Kinase, Fibroblast Growth Factor Receptor 2, and Ephrin Type-A Receptor 5 Kinases in Hepatocellular Carcinoma |
| 2018 | Hepatology | 13.5 | Sphingosine kinase 1 promotes liver fibrosis by preventing miR-19b-3p-mediated inhibition of CCR2 |
| 2018 | Cell Host & Microbe | 30.3 | Listeria Adhesion Protein Induces Intestinal Epithelial Barrier Dysfunction for Bacterial Translocation |
| 2018 | Cell | 64.5 | CD10+ GPR77+ cancer-associated fibroblasts promote cancer formation and chemoresistance by sustaining cancer stemness |
| 2019 | Theranostics | 12.4 | Monoclonal Antibody againstCXCL1 (HL2401) as a Novel Agent in Suppressing IL6 Expression and Tumoral Growth |
| 2019 | Neurology | 9.9 | Changes in cerebralautoregulation and blood biomarkers after remote ischemic preconditioning |
| 2019 | Nature Communications | 16.6 | Altered respiratory virome and serum cytokine profile associated with recurrent respiratory tract infections in children |
| 2019 | Nature Communications | 16.6 | Microenvironmental IL1β promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling |
| 2019 | Nature Cell Biology | 21.3 | NAD+ metabolism governs the proinflammatory senescence-associated secretome |
| 2019 | Nature Medicine | 82.9 | Stress–glucocorticoid–TSC22D3 axis compromises therapy-induced antitumor immunity |
| 2019 | Cell Research | 44.1 | Sodium oligomannate therapeutically remodels gutmicrobiota and suppresses gut bacterial amino acids-shaped neuroinflammation toinhibit Alzheimer’s disease progression |
| 2019 | Cell | 64.5 | Regional activation of myosin II in cancer cells drives tumor progression via a secretory cross-talk with the immune microenvironment |
| 2020 | Theranostics | 12.4 | Targeting the STING pathway in tumor-associated macrophages regulates innate immune sensing of gastric cancer cells |
| 2020 | Theranostics | 12.4 | Plasma protein-based signature predicts distant metastasis and inductionchemotherapy benefit in Nasopharyngeal Carcinoma |
| 2020 | Molecular Therapy | 12.4 | HLSC-Derived Extracellular Vesicles Attenuate Liver Fibrosis and Inflammation in a Murine Model of Non-alcoholic Steatohepatitis |
| 2020 | Acta Pharmaceutica Sinica B | 14.5 | Chrysin serves as a novel inhibitor of DGKa/FAK interaction to suppress the malignancy of esophageal squamous cellcarcinoma (ESCC) |
| 2020 | Journal of Extracellular Vesicles | 16 | Small extracellular vesicles derived from human mesenchymal stromal cells prevent group 2 innatelymphoid cell-dominant allergic airway inflammation through delivery ofmiR-146a-5p |
| 2020 | Nature Communications | 16.6 | Distinct effects of generalanesthetics on lung metastasis mediated by IL-6/JAK/STAT3 pathway in mouse models |
| 2020 | Molecular Cell | 16.6 | Ectosomal PKM2 Promotes HCC by Inducing Macrophage Differentiation and Remodeling the Tumor Microenvironment |
| 2020 | Immunity | 32.4 | Excessive polyamine generation in keratinocytes promotes self-RNA sensing by dendritic cells in psoriasis |
| 2021 | Pharmacological Research | 9.3 | Affiliated Hospital of Integrated Traditional Chinese and WesternMedicine, Nanjing University of Chinese Medicine |
| 2021 | Theranostics | 12.4 | Intravenous transplantationof olfactory ensheathing cells reduces neuroinflammation after spinal cordinjury via interleukin-1 receptor antagonist |
| 2021 | Theranostics | 12.4 | Tumor suppressor DRD2 facilitates M1 macrophages and restricts NF-κB signaling to trigger pyroptosis in breast cancer |
| 2021 | Hepatology | 13.5 | Group-2 Innate LymphoidCells Promote Hepatocellular Carcinoma Progression via CXCL2-Neutrophil Induced Immunosuppression |
| 2021 | Nature Cell Biology | 21.3 | m6A-independent genome-wide METTL3 and METTL14 redistribution drives the senescence-associated secretory phenotype |
| 2021 | Signal Transduction and Targeted Therapy | 39.3 | Mutant KRAS triggers functional reprogramming of tumor-associated macrophages in colorectal cancer |
| 2021 | Cancer Cell | 50.3 | Three subtypes of lung cancer fibroblasts define distinct therapeutic paradigms |
| 2022 | Journal for ImmunoTherapy of Cancer | 10.9 | Longitudinal plasma proteomic profiling of patients with non-small cell lung cancer undergoing immune checkpoint blockade |
| 2022 | PNAS | 11.1 | Plasma metabolome and cytokine profile reveal glycylproline modulating antibody fading in convalescent COVID-19 patients |
| 2022 | Advanced Science | 15.1 | Mouse Modeling Dissecting Macrophage–BreastCancer Communication Uncovered Roles of PYK2 in Macrophage Recruitment andBreast Tumorigenesis |
| 2022 | Nature Microbiology | 28.3 | Multi-omics analyses of airway host–microbe interactions in chronic obstructive pulmonary disease identify potential therapeutic interventions |
| 2023 | Nature Metabolism | 20.8 | TREM2hi resident macrophages protect the septic heart by maintaining cardiomyocyte homeostasis |
| 2023 | Journal of Hematology & Oncology | 28.5 | LCN2 secreted by tissue-infiltrating neutrophils induces the ferroptosis and wasting of adipose and muscle tissues in lung cancer cachexia |
| 2023 | Cancer cell | 50.3 | Tissue factor is a critical regulator of radiation therapy-induced glioblastoma remodeling |
【泪部资料】蛋白芯片案例集锦 | 肿瘤/癌症专题（一）科研重器15篇
【泪部资料】案例集锦 | 蛋白芯片与抗体芯片在生物医学研究和转化医学中的应用（一）
