世
应用案例1
/机制研究
杂志名称：Science Immunology
影响因子：17.6（2024）
文章题目：PDIA3 epitope-driven immune autoreactivity contributes to hepatic damage in type 2 diabetes.
第一作者：Cristina C. Clement
通讯作者：Laura Santambrogio
作者单位：Department of Radiation Oncology, Weill Cornell Medicine, New York
本实验所用产品：QAM-TH17-1（检测小鼠Th1/Th2/Th17相关的18个因子芯片）&PKM proteins
实验样品类型： 肝脏细胞
研究背景：
高脂肪和高果糖（HFHF）饮食可驱动的脂肪毒性和糖毒性诱导肝损伤，从而促进树突状细胞活化，并产生主要的组织相容性复合体II类(MHC-II)免疫肽丘，富含来自细胞代谢、氧化磷酸化和应激反应的蛋白质的肽。
研究结果：
研究表明，证明了HFHF饮食驱动的脂肪毒性和糖毒性可引起代谢损伤可引起肝损伤，并促进由T和B细胞PDIA3表位驱动的致病性免疫自身反应性，参与免疫原性细胞死亡。
研究思路：
使用QAM-TH17-1分析对照和HFHF饮食小鼠的总肝细胞裂解液中是否含有炎症细胞因子。与对照组相比，HFHF喂养小鼠的pdia3反应细胞分泌的GZMB和多种炎症细胞因子(包括IL-17、IL-23、TNF和IFN-y)的量增加，表明HFHF喂养小鼠的肝脏中存在pdia3特异性T细胞，即使在没有免疫的情况下，对其同源PDIA3肽的增殖也会向功能性TH1和TH17型分化，并释放促炎细胞因子和细胞毒性分子。
应用案例2
/ 药物疗效
杂志名称：EMBO Molecular Medicine
影响因子：9.0（2024）
文章题目：Tumor‐associated macrophages‐educated reparative macrophages promote diabetic wound healing
第一作者：母若雨
通讯作者：董磊，王春明
作者单位：澳门大学，中药质量研究国家重点实验室
本实验所用产品：AAM-BLM-1（可同时半定量检测小鼠308个因子，包含细胞因子、趋化因子、脂肪因子、生长因子、蛋白酶、可溶性受体、可溶性粘附分子等）
实验样品类型：细胞上清
研究结果：
本项研究向我们展示了新型巨噬细胞TAMEMs用于糖尿病伤口愈合的制备，通过细胞及体内实验验证了TAMEMs在小鼠糖尿病伤口中发挥的关键修复活性，包括调节炎症，刺激血管生成和促进增殖。其中利用RayBio 能同时半定量检测308种细胞因子的膜芯片（AAM-BLM-1）一次性地对TAMs – CM中的因子含量进行了检测，快速地锁定了重组蛋白培养基中关键蛋白的含量。
研究思路：
本项研究向我们展示了新型巨噬细胞TAMEMs用于糖尿病伤口愈合的制备，通过细胞及体内实验验证了TAMEMs在小鼠糖尿病伤口中发挥的关键修复活性，包括调节炎症，刺激血管生成和促进增殖。其中利用RayBio 能同时半定量检测308种细胞因子的膜芯片（AAM-BLM-1）一次性地对TAMs – CM中的因子含量进行了检测，快速地锁定了重组蛋白培养基中关键蛋白的含量。
应用案例3
/ 标志物筛选
杂志名称：Aging
影响因子：3.9（2024）
文章题目：Plasma cytokines for predicting diabetic retinopathy among type 2 diabetic patients via machine learning algorithms
第一作者：Bin Cao
通讯作者：Dong Zhao
作者单位：首都医科大学
本实验所用产品：GSH-ANG-2&GSH-ANG-3（同时半定量测60种人血管生成相关细胞因子）
实验样品类型：血液
研究结果：
研究利用RayBio抗体芯片（GSH-ANG-2&GSH-ANG-3）检测出NPDR患者中血浆中ANG-1，PDGF-BB，TIMP-2和VEGFR2的血浆细胞因子增加，并且从血浆细胞因子中基于机器学习算法找到NPDR分类器，即从DM患者中进行对NPDR的预测。血浆细胞因子可能是NPDR的强风险标志物。
研究思路
①选取14例DM病人和14例NPDR病人，通过他们的血液样本进行蛋白芯片（GSH-ANG-2&GSH-ANG-3，共60个蛋白）实验，找到两组间表达的差异蛋白。
通过芯片实验结果每种蛋白的表达量绘制热图、火山图和PCA图等，加以限制条件（FC>4或者FC<0.25）进行筛选差异显著且差异倍数大的蛋白。
②选出的六个差异蛋白PDGF-BB, TIMP-1, TIMP-2, ANG-1, CXCL16和VEGFR2用验证集的大量样本（DM：115；NPDR:115）通过ELISA进行验证。
③用两种算法随机森林和lasso回归找到更为关键的三种蛋白（PDGF-BB、VEGFR2、ANG-1）进行后续的建模。
④通过多种模型（神经网络，逻辑回归，支持向量机，XGBOOST和随机森林）构建然后评估每个模型的准确率。最终找到表现最好的随机森林模型，预测的准确率达到85%。
糖尿病研究领域其他产品应用文章
▼ RayBio®抗体芯片近期部分学术引用
| Year | Journal | 2023 IF | Article Title |
| 2017 | Nature Communications | 16.6 | Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis |
| 2017 | Nature Immunology | 30.5 | Direct control of regulatory T cells by keratinocytes |
| 2017 | Nature | 64.8 | Cytoplasmic chromatin triggers inflammation in senescence and cancer |
| 2017 | Nature Medicines | 82.9 | Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms |
| 2018 | Acta Biomaterialia | 9.7 | Manipulation of the response of human endothelial colony-forming cells by focal adhesion assembly using gradient nanopattern plates |
| 2018 | Journal for Immuno Therapy of Cancer | 10.9 | Randomized phase II trial of autologous dendritic cell vaccines versus autologous tumor cell vaccines in metastatic melanoma 5-year follow up and additional analyses |
| 2018 | Arthritis & Rheumatology | 13.3 | Myogenic Progenitor Cells Exhibit Type I Interferon–Driven Proangiogenic Properties and Molecular Signature During Juvenile Dermatomyositis |
| 2018 | Hepatology | 13.5 | Identification and Therapeutic Intervention of Coactivated Anaplastic Lymphoma Kinase, Fibroblast Growth Factor Receptor 2, and Ephrin Type-A Receptor 5 Kinases in Hepatocellular Carcinoma |
| 2018 | Hepatology | 13.5 | Sphingosine kinase 1 promotes liver fibrosis by preventing miR-19b-3p-mediated inhibition of CCR2 |
| 2018 | Cell Host & Microbe | 30.3 | Listeria Adhesion Protein Induces Intestinal Epithelial Barrier Dysfunction for Bacterial Translocation |
| 2018 | Cell | 64.5 | CD10+ GPR77+ cancer-associated fibroblasts promote cancer formation and chemoresistance by sustaining cancer stemness |
| 2019 | Theranostics | 12.4 | Monoclonal Antibody againstCXCL1 (HL2401) as a Novel Agent in Suppressing IL6 Expression and Tumoral Growth |
| 2019 | Neurology | 9.9 | Changes in cerebralautoregulation and blood biomarkers after remote ischemic preconditioning |
| 2019 | Nature Communications | 16.6 | Altered respiratory virome and serum cytokine profile associated with recurrent respiratory tract infections in children |
| 2019 | Nature Communications | 16.6 | Microenvironmental IL1β promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling |
| 2019 | Nature Cell Biology | 21.3 | NAD+ metabolism governs the proinflammatory senescence-associated secretome |
| 2019 | Nature Medicine | 82.9 | Stress–glucocorticoid–TSC22D3 axis compromises therapy-induced antitumor immunity |
| 2019 | Cell Research | 44.1 | Sodium oligomannate therapeutically remodels gutmicrobiota and suppresses gut bacterial amino acids-shaped neuroinflammation toinhibit Alzheimer’s disease progression |
| 2019 | Cell | 64.5 | Regional activation of myosin II in cancer cells drives tumor progression via a secretory cross-talk with the immune microenvironment |
| 2020 | Theranostics | 12.4 | Targeting the STING pathway in tumor-associated macrophages regulates innate immune sensing of gastric cancer cells |
| 2020 | Theranostics | 12.4 | Plasma protein-based signature predicts distant metastasis and inductionchemotherapy benefit in Nasopharyngeal Carcinoma |
| 2020 | Molecular Therapy | 12.4 | HLSC-Derived Extracellular Vesicles Attenuate Liver Fibrosis and Inflammation in a Murine Model of Non-alcoholic Steatohepatitis |
| 2020 | Acta Pharmaceutica Sinica B | 14.5 | Chrysin serves as a novel inhibitor of DGKa/FAK interaction to suppress the malignancy of esophageal squamous cellcarcinoma (ESCC) |
| 2020 | Journal of Extracellular Vesicles | 16 | Small extracellular vesicles derived from human mesenchymal stromal cells prevent group 2 innatelymphoid cell-dominant allergic airway inflammation through delivery ofmiR-146a-5p |
| 2020 | Nature Communications | 16.6 | Distinct effects of generalanesthetics on lung metastasis mediated by IL-6/JAK/STAT3 pathway in mouse models |
| 2020 | Molecular Cell | 16.6 | Ectosomal PKM2 Promotes HCC by Inducing Macrophage Differentiation and Remodeling the Tumor Microenvironment |
| 2020 | Immunity | 32.4 | Excessive polyamine generation in keratinocytes promotes self-RNA sensing by dendritic cells in psoriasis |
| 2021 | Pharmacological Research | 9.3 | Affiliated Hospital of Integrated Traditional Chinese and WesternMedicine, Nanjing University of Chinese Medicine |
| 2021 | Theranostics | 12.4 | Intravenous transplantationof olfactory ensheathing cells reduces neuroinflammation after spinal cordinjury via interleukin-1 receptor antagonist |
| 2021 | Theranostics | 12.4 | Tumor suppressor DRD2 facilitates M1 macrophages and restricts NF-κB signaling to trigger pyroptosis in breast cancer |
| 2021 | Hepatology | 13.5 | Group-2 Innate LymphoidCells Promote Hepatocellular Carcinoma Progression via CXCL2-Neutrophil Induced Immunosuppression |
| 2021 | Nature Cell Biology | 21.3 | m6A-independent genome-wide METTL3 and METTL14 redistribution drives the senescence-associated secretory phenotype |
| 2021 | Signal Transduction and Targeted Therapy | 39.3 | Mutant KRAS triggers functional reprogramming of tumor-associated macrophages in colorectal cancer |
| 2021 | Cancer Cell | 50.3 | Three subtypes of lung cancer fibroblasts define distinct therapeutic paradigms |
| 2022 | Journal for ImmunoTherapy of Cancer | 10.9 | Longitudinal plasma proteomic profiling of patients with non-small cell lung cancer undergoing immune checkpoint blockade |
| 2022 | PNAS | 11.1 | Plasma metabolome and cytokine profile reveal glycylproline modulating antibody fading in convalescent COVID-19 patients |
| 2022 | Advanced Science | 15.1 | Mouse Modeling Dissecting Macrophage–BreastCancer Communication Uncovered Roles of PYK2 in Macrophage Recruitment and Breast Tumorigenesis |
| 2022 | Nature Microbiology | 28.3 | Multi-omics analyses of airway host–microbe interactions in chronic obstructive pulmonary disease identify potential therapeutic interventions |
| 2023 | Nature Metabolism | 20.8 | TREM2hiresident macrophages protect the septic heart by maintaining cardiomyocyte homeostasis |
| 2023 | Journal of Hematology & Oncology | 28.5 | LCN2 secreted by tissue-infiltrating neutrophils induces the ferroptosis and wasting of adipose and muscle tissues in lung cancer cachexia |
| 2023 | Cancer Cell | 50.3 | Tissue factor is a critical regulator of radiation therapy-induced glioblastoma remodeling |
▼ RayBio®ELISA近期部分学术引用
| Year | Journal | 2023 IF | Article Title |
| 2017 | Science Translational Medicine | 17.1 | Detection of early pancreatic ductal adenocarcinoma using thrombospondin-2 and CA19-9 blood markers |
| 2017 | JAMA Internal Medicine | 39.0 | Association of Ozone Exposure With Cardiorespiratory Pathophysiologic Mechanisms in Healthy Adults |
| 2017 | Journal of Clinical Investigation | 15.9 | Thrombin promotes diet-induced obesity through fibrin-driven inflammation |
| 2017 | Nature Medicine | 82.9 | Targeting CASP8 and FADD-like apoptosis regulator ameliorates nonalcoholic steatohepatitis in mice and nonhuman primates |
| 2017 | Nature Communications | 16.6 | Tyrosine phosphatase SHP2 negatively regulates NLRP3 inflammasome activation via ANT1-dependent mitochondrial homeostasis |
| 2018 | Nature | 64.8 | Disruption of a self-amplifying catecholamine loop reduces cytokine release syndrome |
| 2018 | Autophagy | 13.3 | HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer |
| 2020 | Annals of Surgery | 9.0 | Plasma miR-181a-5p Downregulation Predicts Response and Improved Survival After FOLFIRINOX in Pancreatic Ductal Adenocarcinoma |
| 2020 | Nature Communications | 16.6 | Plasma levels of DPP4 activity and sDPP4 are dissociated from inflammation in mice and humans |
| 2020 | Nature Communications | 16.6 | Chemotaxis-driven delivery ofnano-pathogenoids for co mplete eradication oftumorspost-phototherapy |
| 2020 | Nature Communications | 16.6 | Receptor-targetedengineered probiotics mitigatele thal Listeria infection |
| 2020 | Brain Behavior and Immunity | 15.1 | Increased vulnerability to impulsive behavior after streptococcal antigen exposure and antibiotic treatment in rats |
| 2020 | Cell Metabolism | 29.0 | Defined p16High Senescent Cell Types Are Indispensablefor Mouse Healthspan |
| 2020 | Immunity | 32.4 | Interleukin-33 Promotes Serotonin Release from Enterochromaffin Cells for Intestinal Homeostasis |
| 2020 | Cell | 64.5 | Mortality Risk Profiling of Staphylococcus aureus Bacteremia by Multi-omic Serum Analysis Reveals Early Predictive and Pathogenic Signatures |
| 2021 | Journal of Clinical Investigation | 15.9 | Mutant SF3B1 promotes AKT-and NF-KB-driven mammary tumorigenesis |
| 2022 | Biomaterials | 14.0 | Dual-functionalhydrogel systemfor spinal cordr egeneration with sustained releaseofarylsulfataseBalleviatesfibrotic mi croenvironment and promotes axonalregeneration |
【泪部资料】蛋白芯片案例集锦 | 肿瘤/癌症专题（一）科研重器15篇
【泪部资料】案例集锦 | 蛋白芯片与抗体芯片在生物医学研究和转化医学中的应用（一）
