抗体芯片在病毒学研究中的应用
杂志名称：
Journal of Infection（2023 IF：28.2）
文献题目：
Comparison between human infections caused by highly and low pathogenic H7N9 avian influenza viruses in Wave Five: clinical and virological findings
第一作者：
Yang Yang
通讯作者：
Gao George F 等
作者单位：
深圳市病原体与免疫深圳市重点实验室
广东省新发传染病诊治重点实验室，传染病国家重点学科
南方科技大学附属第二医院深圳市第三人民医院中国科学院流感研究与预警中心微生物研究所
中国科学院病原微生物学与免疫学重点实验室，传染病诊疗协同创新中心
发表时间：
2019年
文献全文下载地址：
暂无免费下载资源，若需原文可私信索取
本实验所用产品：
品名：Human Cytokine Array G5
货号：AAH-CYT-G5（人80个细胞因子半定量抗体芯片）
详情：https://www.raybiotech.com/human-cytokine-array-g5-aah-cyt-g5
因子列表：
芯片类型：
研究目的
新出现的高致病性(HP) H7N9禽流感病毒与（LP）低致病性H7N9病毒相比，人感染禽流感病毒的病死率和耐药流行率更高，文章对第五波期间新出现的HP-H7N9和LP-H7N9进行分析，以帮助临床快速准确地对禽流感病人进行病毒感染株型确定及治疗。
思路&结果
1、测定12个病人的H7N9病毒分型
HP-H7N9和LP-H7N9感染在年龄、发病至住院天数、共存慢性疾病和住院期间并发症发生率方面无显著差异，但在分离的病毒PB2片段中存在氨基酸突变。
数据库分析结果，H7N9病毒PB2片段的E627K和D701N突变与小鼠和白鼬的传播有关，K526R突变与增强与K627和N701功能的有关。
2、12个病人的临床检测
LP-组的白细胞减少和血小板减少比较明显，其余指标差别都不大。
3、AAH-CYT-G5测定病人血清的细胞因子和趋化因子
细胞因子与疾病严重程度相关：与健康对照组相比，LP组和HP组的MIF、MCP-1和IP-10等抗病毒感染等细胞因子均显著升高。
4、利用血气分析仪计算并分析病人的缺氧与肺损伤程度
H7N9患者入院后有严重肺部疾病，并具有ARDS疾病的特征。两组患者PaO2/FiO2动态模式相似，说明两种病毒引起的疾病严重程度相似。
5、病人病毒载量分析
H7N9病毒PB2片段的E627K和D701N突变与小鼠和白鼬的传播有关，K526R突变与增强与K627和N701功能的有关。
讨论 & 结论
Discussion & Conclusion
实验结果表明：HP-H7N9和LP-H7N9感染的人群在临床表征，蛋白水平上在患者入院时差异都不大，所以需要经过感染病毒测序区别感染的株型，从而进行相应的治疗。
Tips：
① 这篇文章中研究者用了RayBio AAH-CYT-G5（人类80个因子）芯片检测了三组人群，在满足统计学的情况下测9个样本，试剂盒总计2W左右，最迟1个月出结果。
② 如果老师选择做WB检测流感病毒免疫相关的蛋白，购买所有抗体需花费16个W，做完80个因子的WB要花的时间为，昂~报说！反正做完WB流感病毒说不定已经变异好几代了！
③ 综上，选择抗体芯片作为研究工具，可以快速、省时、省力、省资金地出研究数据发高分文章！
④ 其他热门研究专题小芯片推荐，私信或致电020-29891010详询：
⑤ 目前活动期间，针对120个因子以内的夹心法芯片有特殊优惠，以以上文章为例，可以节省约1.4w。点击链接，了解更多优惠活动…
▼ RayBio®抗体芯片近期部分学术引用
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