免疫研究，作为2023年国自然中标数量最多的热门研究方向，和细胞信号通路JAK-STAT的关系非常密切。除了免疫，JAK/STAT与许多炎症、遗传疾病、肿瘤也有密切的相关性，在临床科学研究中，无论是机制研究还是治疗相关药物的筛选，都受到越来越多的人的关注，可谓是永不消逝的免疫相关信号，相信2024年的研究热度也不会降低。
JAK/STAT信号通路是一种广泛表达的细胞内信号转导通路，调控着免疫、分化、凋亡、增殖等多种细胞生物学过程[1]。涉及的关键酶包含Janus激酶(JAKs)家族、信号转导和转录激活因子(STAT)蛋白家族、细胞因子信号传导/细胞因子诱导Src同源2 (SH2)-containing (SOCS/CIS)蛋白家族的抑制因子等[2]。
JAK/STAT分子通路
JAK/STAT的活化起始于细胞因子与受体的结合，招募Jak发生磷酸化，再通过SH2的介导使得STATs发生酪氨酸磷酸化并转移至细胞核中，激活特定基因的转录。
Liang, Dong et al. “JAK/STAT in leukemia: a clinical update.” Molecular cancer vol. 23,1 25. 26 Jan. 2024
JAK/STAT在疾病中作用
大量研究已经表明，JAK/STAT信号通路与多种疾病的发生和发展有密切关系，激活JAK/STAT通路的细胞因子包含白介素、干扰素、生长因子等家族最终影响基因的表达，所以JAK/STAT可参与炎症免疫相关疾病及癌症的调节。
自身免疫性疾病
在某些自身免疫性疾病中，如系统性红斑狼疮[3]和银屑病[4]，JAK/STAT信号通路的异常激活与疾病的活动度和严重程度密切相关。一些先天性的免疫疾病也与JAK/STAT信号失调相关此外，JAK抑制剂也可作为这些疾病的治疗方案及媒介[5]。当机体内免疫系统失调会导致JAK/STAT通路崩溃，影响后续的基因表达，驱动T细胞白血病/淋巴瘤的发生[6]。
炎症疾病
例如类风湿性关节炎、皮肤炎症、肠炎等，早有研究表明JAK抑制剂可在风湿病中发挥作用[7]，研究发现阻断IL-6信号可以有效抑制一些慢性炎症[8]。
癌症
在许多研究中表明，JAK/STAT通路蛋白的突变，可以导致下游基因表达激活，导致癌症产生，例如STAT3在许多癌症（(膀胱癌、乳腺癌、神经癌症、结肠癌和宫颈癌等）中被激活[9]。
······
目前虽然有tofacitinib和baricitinib等抑制剂的获批，但是其副作用和安全性的问题也还未解决，所以目前对于JAK/STAT通路相关抑制剂的研究也有非常大的空间[10]。
如何快速研究JAK/STAT通路中细胞因子及下游疾病的关系？
RayBio JAK/STAT通路功能性微量表达蛋白多因子检测试剂盒，涵盖JAK/STAT相关152个细胞因子，可以从上游细胞因子、JAK/STAT通路因子、下游调控蛋白翻译情况多个维度评估实验样本。
可用于探究JAK/STAT相关的疾病机制、疾病抑制剂、治疗方案等方面的全面研究。
标红靶标为试剂盒涵盖的JAK/STAT部分检测因子
JAK/STAT 通路探究专用试剂盒
货号：AAH-BLG-JAK
检测因子数量：152（JAK/STAT通路上下游微量表达的功能性细胞因子）
技术原理：荧光标记抗原抗体结合
可选载体：载玻片
检测样本：人类血清、血浆、细胞上清、细胞裂解物、组织裂解物……
检测仪器：玻璃芯片扫描仪（Cy3荧光、分辨率≤20μm）
检测样本用量（参考，具体可以结合实际情况调整）
血清/血浆：100μL
细胞上清等无需浓缩的液体样本：1mL
细胞上清/洗液等需浓缩液体样本：10mL
组织/细胞裂解蛋白量：30μg
JAK/STAT 专用试剂盒联合检测方向
▼ RayBio®抗体芯片近期部分学术引用
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| 2017 | Nature Communications | 16.6 | Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis |
| 2017 | Nature Immunology | 30.5 | Direct control of regulatory T cells by keratinocytes |
| 2017 | Nature | 64.8 | Cytoplasmic chromatin triggers inflammation in senescence and cancer |
| 2017 | Nature Medicines | 82.9 | Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms |
| 2018 | Acta Biomaterialia | 9.7 | Manipulation of the response of human endothelial colony-forming cells by focal adhesion assembly using gradient nanopattern plates |
| 2018 | Journal for Immuno Therapy of Cancer | 10.9 | Randomized phase II trial of autologous dendritic cell vaccines versus autologous tumor cell vaccines in metastatic melanoma 5-year follow up and additional analyses |
| 2018 | Arthritis & Rheumatology | 13.3 | Myogenic Progenitor Cells Exhibit Type I Interferon–Driven Proangiogenic Properties and Molecular Signature During Juvenile Dermatomyositis |
| 2018 | Hepatology | 13.5 | Identification and Therapeutic Intervention of Coactivated Anaplastic Lymphoma Kinase, Fibroblast Growth Factor Receptor 2, and Ephrin Type-A Receptor 5 Kinases in Hepatocellular Carcinoma |
| 2018 | Hepatology | 13.5 | Sphingosine kinase 1 promotes liver fibrosis by preventing miR-19b-3p-mediated inhibition of CCR2 |
| 2018 | Cell Host & Microbe | 30.3 | Listeria Adhesion Protein Induces Intestinal Epithelial Barrier Dysfunction for Bacterial Translocation |
| 2018 | Cell | 64.5 | CD10+ GPR77+ cancer-associated fibroblasts promote cancer formation and chemoresistance by sustaining cancer stemness |
| 2019 | Theranostics | 12.4 | Monoclonal Antibody againstCXCL1 (HL2401) as a Novel Agent in Suppressing IL6 Expression and Tumoral Growth |
| 2019 | Neurology | 9.9 | Changes in cerebralautoregulation and blood biomarkers after remote ischemic preconditioning |
| 2019 | Nature Communications | 16.6 | Altered respiratory virome and serum cytokine profile associated with recurrent respiratory tract infections in children |
| 2019 | Nature Communications | 16.6 | Microenvironmental IL1β promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling |
| 2019 | Nature Cell Biology | 21.3 | NAD+ metabolism governs the proinflammatory senescence-associated secretome |
| 2019 | Nature Medicine | 82.9 | Stress–glucocorticoid–TSC22D3 axis compromises therapy-induced antitumor immunity |
| 2019 | Cell Research | 44.1 | Sodium oligomannate therapeutically remodels gutmicrobiota and suppresses gut bacterial amino acids-shaped neuroinflammation toinhibit Alzheimer’s disease progression |
| 2019 | Cell | 64.5 | Regional activation of myosin II in cancer cells drives tumor progression via a secretory cross-talk with the immune microenvironment |
| 2020 | Theranostics | 12.4 | Targeting the STING pathway in tumor-associated macrophages regulates innate immune sensing of gastric cancer cells |
| 2020 | Theranostics | 12.4 | Plasma protein-based signature predicts distant metastasis and inductionchemotherapy benefit in Nasopharyngeal Carcinoma |
| 2020 | Molecular Therapy | 12.4 | HLSC-Derived Extracellular Vesicles Attenuate Liver Fibrosis and Inflammation in a Murine Model of Non-alcoholic Steatohepatitis |
| 2020 | Acta Pharmaceutica Sinica B | 14.5 | Chrysin serves as a novel inhibitor of DGKa/FAK interaction to suppress the malignancy of esophageal squamous cellcarcinoma (ESCC) |
| 2020 | Journal of Extracellular Vesicles | 16 | Small extracellular vesicles derived from human mesenchymal stromal cells prevent group 2 innatelymphoid cell-dominant allergic airway inflammation through delivery ofmiR-146a-5p |
| 2020 | Nature Communications | 16.6 | Distinct effects of generalanesthetics on lung metastasis mediated by IL-6/JAK/STAT3 pathway in mouse models |
| 2020 | Molecular Cell | 16.6 | Ectosomal PKM2 Promotes HCC by Inducing Macrophage Differentiation and Remodeling the Tumor Microenvironment |
| 2020 | Immunity | 32.4 | Excessive polyamine generation in keratinocytes promotes self-RNA sensing by dendritic cells in psoriasis |
| 2021 | Pharmacological Research | 9.3 | Affiliated Hospital of Integrated Traditional Chinese and WesternMedicine, Nanjing University of Chinese Medicine |
| 2021 | Theranostics | 12.4 | Intravenous transplantationof olfactory ensheathing cells reduces neuroinflammation after spinal cordinjury via interleukin-1 receptor antagonist |
| 2021 | Theranostics | 12.4 | Tumor suppressor DRD2 facilitates M1 macrophages and restricts NF-κB signaling to trigger pyroptosis in breast cancer |
| 2021 | Hepatology | 13.5 | Group-2 Innate LymphoidCells Promote Hepatocellular Carcinoma Progression via CXCL2-Neutrophil Induced Immunosuppression |
| 2021 | Nature Cell Biology | 21.3 | m6A-independent genome-wide METTL3 and METTL14 redistribution drives the senescence-associated secretory phenotype |
| 2021 | Signal Transduction and Targeted Therapy | 39.3 | Mutant KRAS triggers functional reprogramming of tumor-associated macrophages in colorectal cancer |
| 2021 | Cancer Cell | 50.3 | Three subtypes of lung cancer fibroblasts define distinct therapeutic paradigms |
| 2022 | Journal for ImmunoTherapy of Cancer | 10.9 | Longitudinal plasma proteomic profiling of patients with non-small cell lung cancer undergoing immune checkpoint blockade |
| 2022 | PNAS | 11.1 | Plasma metabolome and cytokine profile reveal glycylproline modulating antibody fading in convalescent COVID-19 patients |
| 2022 | Advanced Science | 15.1 | Mouse Modeling Dissecting Macrophage–BreastCancer Communication Uncovered Roles of PYK2 in Macrophage Recruitment and Breast Tumorigenesis |
| 2022 | Nature Microbiology | 28.3 | Multi-omics analyses of airway host–microbe interactions in chronic obstructive pulmonary disease identify potential therapeutic interventions |
| 2023 | Nature Metabolism | 20.8 | TREM2hiresident macrophages protect the septic heart by maintaining cardiomyocyte homeostasis |
| 2023 | Journal of Hematology & Oncology | 28.5 | LCN2 secreted by tissue-infiltrating neutrophils induces the ferroptosis and wasting of adipose and muscle tissues in lung cancer cachexia |
| 2023 | Cancer Cell | 50.3 | Tissue factor is a critical regulator of radiation therapy-induced glioblastoma remodeling |
▼ RayBio®ELISA近期部分学术引用
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| 2017 | Science Translational Medicine | 17.1 | Detection of early pancreatic ductal adenocarcinoma using thrombospondin-2 and CA19-9 blood markers |
| 2017 | JAMA Internal Medicine | 39.0 | Association of Ozone Exposure With Cardiorespiratory Pathophysiologic Mechanisms in Healthy Adults |
| 2017 | Journal of Clinical Investigation | 15.9 | Thrombin promotes diet-induced obesity through fibrin-driven inflammation |
| 2017 | Nature Medicine | 82.9 | Targeting CASP8 and FADD-like apoptosis regulator ameliorates nonalcoholic steatohepatitis in mice and nonhuman primates |
| 2017 | Nature Communications | 16.6 | Tyrosine phosphatase SHP2 negatively regulates NLRP3 inflammasome activation via ANT1-dependent mitochondrial homeostasis |
| 2018 | Nature | 64.8 | Disruption of a self-amplifying catecholamine loop reduces cytokine release syndrome |
| 2018 | Autophagy | 13.3 | HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer |
| 2020 | Annals of Surgery | 9.0 | Plasma miR-181a-5p Downregulation Predicts Response and Improved Survival After FOLFIRINOX in Pancreatic Ductal Adenocarcinoma |
| 2020 | Nature Communications | 16.6 | Plasma levels of DPP4 activity and sDPP4 are dissociated from inflammation in mice and humans |
| 2020 | Nature Communications | 16.6 | Chemotaxis-driven delivery ofnano-pathogenoids for co mplete eradication oftumorspost-phototherapy |
| 2020 | Nature Communications | 16.6 | Receptor-targetedengineered probiotics mitigatele thal Listeria infection |
| 2020 | Brain Behavior and Immunity | 15.1 | Increased vulnerability to impulsive behavior after streptococcal antigen exposure and antibiotic treatment in rats |
| 2020 | Cell Metabolism | 29.0 | Defined p16High Senescent Cell Types Are Indispensablefor Mouse Healthspan |
| 2020 | Immunity | 32.4 | Interleukin-33 Promotes Serotonin Release from Enterochromaffin Cells for Intestinal Homeostasis |
| 2020 | Cell | 64.5 | Mortality Risk Profiling of Staphylococcus aureus Bacteremia by Multi-omic Serum Analysis Reveals Early Predictive and Pathogenic Signatures |
| 2021 | Journal of Clinical Investigation | 15.9 | Mutant SF3B1 promotes AKT-and NF-KB-driven mammary tumorigenesis |
| 2022 | Biomaterials | 14.0 | Dual-functionalhydrogel systemfor spinal cordr egeneration with sustained releaseofarylsulfataseBalleviatesfibrotic mi croenvironment and promotes axonalregeneration |
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