往期回顾：【microRNA】封神之路（一）| 转录组学 × microRNA
前面，我们已经回顾了miRNA的起源以及miRNA封神之路中与转录组学互相成全的故事。今天，我们要开启一个关于miRNA的新专题——
如你所知：抗体芯片主要用于蛋白检测。表面上看，似乎抗体芯片直接用于miRNA研究有碰瓷嫌疑，实则不然——一方面，miRNA主要功能便是“基因表达调控”，无论是在细胞质中表现出来的负向调控，还是在细胞器（如线粒体、细胞核等）表现出的定位和激活功能，最终都将或多或少体现在蛋白表达变化上，miRNA通过调控蛋白表达来影响细胞功能和疾病进程的研究本来就是miRNA研究的重要内容之一，以蛋白检测为强项的抗体芯片自然而然成为miRNA的一件封神利器！另一方面，“多组学”联合分析无与伦比的意义已经被充分证明，基因和蛋白是生命现象的两个重要维度，而miRNA是联结两个维度的神秘纽带，miRNA上下游的协同研究、多组学联合分析无与伦比的意义无需赘述，于是，对反贼深恶痛绝的小编仰天长啸——MAGA（Make Antibody array Great Again，让抗体芯片再次伟大）！
那么，问题来了——能不能举几个真实的栗子？O了，安排！
研究案例1
杂志名称：J Clin Invest
影响因子：13.3/Q1
文章题目：microRNA-21-5p dysregulation in exosomes derived from heart failure patients impairs regenerative potential
单位：石家庄医科大学第二医院心内科
本实验所用产品：AAH-AKT-1(检测人和小鼠AKT通路中18个热门细胞因子氨基酸磷酸化程度)
实验样本：细胞裂解液
研究目的：miR-21-5p在外泌体介导的心力衰竭心脏修复治疗研究中的修复特性及机制探究。
研究结果：心力衰竭病理状态改变了心源性外泌体的miRNA表达，miR-21-5p通过磷酸酶和紧张素同源物/Akt途径促进血管生成和心肌细胞存活，从而参与外泌体介导的心脏修复。
在确定miR-21-5p对磷酸酶和紧张素同源物/Akt信号传导的影响及潜在的靶基因的实验中，将miR-21-5p转染心肌细胞、CFs、内皮细胞，利用AAH-AKT-1芯片检测18个热门AKT相关磷酸化位点，发现在心肌细胞、内皮细胞PTEN、AKT和BAD是miR -21调控的基因。结合WB实验验证及补充分析，表明miR-21通过靶向PDCD4抑制心肌细胞凋亡，通过激活PTEN/Akt信号通路促进血管生成，促进内皮细胞中VEGF的表达。
研究案例2
杂志名称：Cell Death Differ.
影响因子：13.3/Q1
文章题目：miR-125a-5p attenuates macrophage-mediated vascular dysfunction by targeting Ninjurin1
单位：韩国成均馆大学药学院
本实验所用产品：AAM-CYT-1000（检测小鼠样本中96个炎症、免疫、血管生成等相关的微量表达功能性细胞因子）
实验样本：细胞上清
研究目的：探讨Ninjurin1 （Ninj1）这种具有调节巨噬细胞在透明体退化、多发性硬化和动脉粥样硬化中的功能的粘附分子的生物学相关性及其在血管网络中的作用机制。
研究结果：研究中找到了miR-125a-5p对Ninj1表达有较深的抑制作用，利用AAM-CYT-1000检测过表达RAW 264.7巨噬细胞转染miR-125a-5p对Ninj1在促炎蛋白和促血管生成因子的表达影响中发现，miR-125a-5p对Ninj1有明显的抑制作用。后续可以miR-125a-5p为媒介，通过调控Ninj1血管生成和炎症作为糖尿病视网膜病变的治疗靶点。
研究案例3
杂志名称：Cell Communication and Signaling
影响因子：8.2/Q1
文章题目：The role of miR-155-5p in inflammation and mechanical loading during intervertebral disc degeneration
单位：罗切斯特理工学院生物医学工程系
本实验所用产品：
GSH-CYT-1（检测人类样本中20个炎症、趋化相关细胞因子表达程度）
GSH-MMP-1（检测人类样本中10个金属蛋白酶相关细胞因子表达程度）
AAH-MAPK-1（检测人或小鼠样本中17个热门MAPK上下游相关氨基酸磷酸化位点表达程度）
实验样本：细胞上清及细胞裂解液
研究目的：miR-155-5p被认为是一种促炎介质，是癌症和骨关节炎等疾病的促成因素，本研究旨在揭示miR-155-5p在椎间盘(IVD)退变中炎症及机械传感方面的功能。
研究结论：AF细胞中miR-155-5p表达在机械外力及炎症作用下导致了IL-8、IL-6的上调，miR-155-5p抑制可导致抗炎细胞因子IL-10升高；NP细胞中细胞中miR-155-5p功能性获得可通过增加p38和p53的磷酸化激活MAPK通路，抑制导致TIMP-4显著升高
研究结果1：发现在炎症环境中，miR-155-5p在IVD变性中发挥促炎作用。
miR-155-5p抑制剂及模拟物转染至退行性IVD患者中分离出来的髓核(NP)和纤维环(AF)细胞，利用GSH-CYT-1芯片，检测细胞上清中20个炎症趋化相关的靶标，观察其在炎症中的分泌表型。
研究结果2：前面研究发现，miR-155-5p表达引起的一些促炎细胞因子增加，由于炎症和ECM降解之间的密切联系，研究员对miR-155-5p表达对MMPs及其抑制剂(金属蛋白酶组织抑制剂，TIMPs)的影响，利用了GSH-MMP-1检测了细胞上清中10个金属蛋白酶相关的靶标，发现miR-155-5p抑制后，多种MMP相关的酶存在差异性表达，其中包含MMP-2、9、3 ，TIMP4表达增加。
研究结果3：IVD变性中MAPK、NF-kB和PI3/Akt等细胞内信号通路传递是失调的，其中MAPK通路中ERK， JNK和p38因子在IVD变性中普遍存在，为了确定前面的炎症因子表达增加是否通过MAPK介导，利用AAH-MAPK-1芯片检测miR-155-5p表达对MAPK通路各个热门磷酸化位点的影响，发现miR-155-5p增强了mTOR、ERK1/2、p38等磷酸化程度，表明miR-155-5p可能通过调节MAPK信号通路，导致下游细胞炎症等反应。
研究结果4：由于IVD稳态也与机械载荷和组织承受压缩载荷的能力相关，为了研究miR-155-5p的表达和抑制对力学感知的影响，研究中制作了机械拉伸相关的测量模型，对细胞进行拉伸后进行检测发现COX-2、IL-8等促炎因子显著表达。
确定机械负荷与炎症反应中的关系后，研究者利用GSH-CYT-1芯片设计检测了机械负荷过程中miR-155-5p的功能获得/缺失对细胞因子分泌的影响，发现在周期性拉伸过程中miR-155-5p的表达导致促炎细胞因子IL-6和IL-8的增加，而抑制其表达可以减少IL-1β的分泌。
RayBio拥有多种适用于miRNA研究的高效工具，并可提供一站式高品质技术服务，包括：
① 抗体芯片 ② ELISA试剂盒 ③ 抗体……
若有所托，我们必当全力以赴！
▼ RayBio®抗体芯片近期部分学术引用
| Year | Journal | 2023 IF | Article Title |
| 2017 | Nature Communications | 16.6 | Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis |
| 2017 | Nature Immunology | 30.5 | Direct control of regulatory T cells by keratinocytes |
| 2017 | Nature | 64.8 | Cytoplasmic chromatin triggers inflammation in senescence and cancer |
| 2017 | Nature Medicines | 82.9 | Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms |
| 2018 | Acta Biomaterialia | 9.7 | Manipulation of the response of human endothelial colony-forming cells by focal adhesion assembly using gradient nanopattern plates |
| 2018 | Journal for Immuno Therapy of Cancer | 10.9 | Randomized phase II trial of autologous dendritic cell vaccines versus autologous tumor cell vaccines in metastatic melanoma 5-year follow up and additional analyses |
| 2018 | Arthritis & Rheumatology | 13.3 | Myogenic Progenitor Cells Exhibit Type I Interferon–Driven Proangiogenic Properties and Molecular Signature During Juvenile Dermatomyositis |
| 2018 | Hepatology | 13.5 | Identification and Therapeutic Intervention of Coactivated Anaplastic Lymphoma Kinase, Fibroblast Growth Factor Receptor 2, and Ephrin Type-A Receptor 5 Kinases in Hepatocellular Carcinoma |
| 2018 | Hepatology | 13.5 | Sphingosine kinase 1 promotes liver fibrosis by preventing miR-19b-3p-mediated inhibition of CCR2 |
| 2018 | Cell Host & Microbe | 30.3 | Listeria Adhesion Protein Induces Intestinal Epithelial Barrier Dysfunction for Bacterial Translocation |
| 2018 | Cell | 64.5 | CD10+ GPR77+ cancer-associated fibroblasts promote cancer formation and chemoresistance by sustaining cancer stemness |
| 2019 | Theranostics | 12.4 | Monoclonal Antibody againstCXCL1 (HL2401) as a Novel Agent in Suppressing IL6 Expression and Tumoral Growth |
| 2019 | Neurology | 9.9 | Changes in cerebralautoregulation and blood biomarkers after remote ischemic preconditioning |
| 2019 | Nature Communications | 16.6 | Altered respiratory virome and serum cytokine profile associated with recurrent respiratory tract infections in children |
| 2019 | Nature Communications | 16.6 | Microenvironmental IL1β promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling |
| 2019 | Nature Cell Biology | 21.3 | NAD+ metabolism governs the proinflammatory senescence-associated secretome |
| 2019 | Nature Medicine | 82.9 | Stress–glucocorticoid–TSC22D3 axis compromises therapy-induced antitumor immunity |
| 2019 | Cell Research | 44.1 | Sodium oligomannate therapeutically remodels gutmicrobiota and suppresses gut bacterial amino acids-shaped neuroinflammation toinhibit Alzheimer’s disease progression |
| 2019 | Cell | 64.5 | Regional activation of myosin II in cancer cells drives tumor progression via a secretory cross-talk with the immune microenvironment |
| 2020 | Theranostics | 12.4 | Targeting the STING pathway in tumor-associated macrophages regulates innate immune sensing of gastric cancer cells |
| 2020 | Theranostics | 12.4 | Plasma protein-based signature predicts distant metastasis and inductionchemotherapy benefit in Nasopharyngeal Carcinoma |
| 2020 | Molecular Therapy | 12.4 | HLSC-Derived Extracellular Vesicles Attenuate Liver Fibrosis and Inflammation in a Murine Model of Non-alcoholic Steatohepatitis |
| 2020 | Acta Pharmaceutica Sinica B | 14.5 | Chrysin serves as a novel inhibitor of DGKa/FAK interaction to suppress the malignancy of esophageal squamous cellcarcinoma (ESCC) |
| 2020 | Journal of Extracellular Vesicles | 16 | Small extracellular vesicles derived from human mesenchymal stromal cells prevent group 2 innatelymphoid cell-dominant allergic airway inflammation through delivery ofmiR-146a-5p |
| 2020 | Nature Communications | 16.6 | Distinct effects of generalanesthetics on lung metastasis mediated by IL-6/JAK/STAT3 pathway in mouse models |
| 2020 | Molecular Cell | 16.6 | Ectosomal PKM2 Promotes HCC by Inducing Macrophage Differentiation and Remodeling the Tumor Microenvironment |
| 2020 | Immunity | 32.4 | Excessive polyamine generation in keratinocytes promotes self-RNA sensing by dendritic cells in psoriasis |
| 2021 | Pharmacological Research | 9.3 | Affiliated Hospital of Integrated Traditional Chinese and WesternMedicine, Nanjing University of Chinese Medicine |
| 2021 | Theranostics | 12.4 | Intravenous transplantationof olfactory ensheathing cells reduces neuroinflammation after spinal cordinjury via interleukin-1 receptor antagonist |
| 2021 | Theranostics | 12.4 | Tumor suppressor DRD2 facilitates M1 macrophages and restricts NF-κB signaling to trigger pyroptosis in breast cancer |
| 2021 | Hepatology | 13.5 | Group-2 Innate LymphoidCells Promote Hepatocellular Carcinoma Progression via CXCL2-Neutrophil Induced Immunosuppression |
| 2021 | Nature Cell Biology | 21.3 | m6A-independent genome-wide METTL3 and METTL14 redistribution drives the senescence-associated secretory phenotype |
| 2021 | Signal Transduction and Targeted Therapy | 39.3 | Mutant KRAS triggers functional reprogramming of tumor-associated macrophages in colorectal cancer |
| 2021 | Cancer Cell | 50.3 | Three subtypes of lung cancer fibroblasts define distinct therapeutic paradigms |
| 2022 | Journal for ImmunoTherapy of Cancer | 10.9 | Longitudinal plasma proteomic profiling of patients with non-small cell lung cancer undergoing immune checkpoint blockade |
| 2022 | PNAS | 11.1 | Plasma metabolome and cytokine profile reveal glycylproline modulating antibody fading in convalescent COVID-19 patients |
| 2022 | Advanced Science | 15.1 | Mouse Modeling Dissecting Macrophage–BreastCancer Communication Uncovered Roles of PYK2 in Macrophage Recruitment and Breast Tumorigenesis |
| 2022 | Nature Microbiology | 28.3 | Multi-omics analyses of airway host–microbe interactions in chronic obstructive pulmonary disease identify potential therapeutic interventions |
| 2023 | Nature Metabolism | 20.8 | TREM2hiresident macrophages protect the septic heart by maintaining cardiomyocyte homeostasis |
| 2023 | Journal of Hematology & Oncology | 28.5 | LCN2 secreted by tissue-infiltrating neutrophils induces the ferroptosis and wasting of adipose and muscle tissues in lung cancer cachexia |
| 2023 | Cancer Cell | 50.3 | Tissue factor is a critical regulator of radiation therapy-induced glioblastoma remodeling |
▼ RayBio®ELISA近期部分学术引用
| Year | Journal | 2023 IF | Article Title |
| 2017 | Science Translational Medicine | 17.1 | Detection of early pancreatic ductal adenocarcinoma using thrombospondin-2 and CA19-9 blood markers |
| 2017 | JAMA Internal Medicine | 39.0 | Association of Ozone Exposure With Cardiorespiratory Pathophysiologic Mechanisms in Healthy Adults |
| 2017 | Journal of Clinical Investigation | 15.9 | Thrombin promotes diet-induced obesity through fibrin-driven inflammation |
| 2017 | Nature Medicine | 82.9 | Targeting CASP8 and FADD-like apoptosis regulator ameliorates nonalcoholic steatohepatitis in mice and nonhuman primates |
| 2017 | Nature Communications | 16.6 | Tyrosine phosphatase SHP2 negatively regulates NLRP3 inflammasome activation via ANT1-dependent mitochondrial homeostasis |
| 2018 | Nature | 64.8 | Disruption of a self-amplifying catecholamine loop reduces cytokine release syndrome |
| 2018 | Autophagy | 13.3 | HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer |
| 2020 | Annals of Surgery | 9.0 | Plasma miR-181a-5p Downregulation Predicts Response and Improved Survival After FOLFIRINOX in Pancreatic Ductal Adenocarcinoma |
| 2020 | Nature Communications | 16.6 | Plasma levels of DPP4 activity and sDPP4 are dissociated from inflammation in mice and humans |
| 2020 | Nature Communications | 16.6 | Chemotaxis-driven delivery ofnano-pathogenoids for co mplete eradication oftumorspost-phototherapy |
| 2020 | Nature Communications | 16.6 | Receptor-targetedengineered probiotics mitigatele thal Listeria infection |
| 2020 | Brain Behavior and Immunity | 15.1 | Increased vulnerability to impulsive behavior after streptococcal antigen exposure and antibiotic treatment in rats |
| 2020 | Cell Metabolism | 29.0 | Defined p16High Senescent Cell Types Are Indispensablefor Mouse Healthspan |
| 2020 | Immunity | 32.4 | Interleukin-33 Promotes Serotonin Release from Enterochromaffin Cells for Intestinal Homeostasis |
| 2020 | Cell | 64.5 | Mortality Risk Profiling of Staphylococcus aureus Bacteremia by Multi-omic Serum Analysis Reveals Early Predictive and Pathogenic Signatures |
| 2021 | Journal of Clinical Investigation | 15.9 | Mutant SF3B1 promotes AKT-and NF-KB-driven mammary tumorigenesis |
| 2022 | Biomaterials | 14.0 | Dual-functionalhydrogel systemfor spinal cordr egeneration with sustained releaseofarylsulfataseBalleviatesfibrotic mi croenvironment and promotes axonalregeneration |
【泪部资料】蛋白芯片案例集锦 | 肿瘤/癌症专题（一）科研重器15篇
【泪部资料】案例集锦 | 蛋白芯片与抗体芯片在生物医学研究和转化医学中的应用（一）
