炎症
炎症是指活体组织对各种损伤因子的刺激所发生的以防御反应（损伤、抗损伤和修复）为主的基本病理动态过程主要表现为红、肿、热、痛及功能障碍。
炎症是日常生活当中最常见的疾病，有急性炎症，也有慢性炎症，有胃炎，鼻炎，牙周炎，肠道炎，咽喉炎，前列腺炎，阴道炎，宫颈炎，肩周炎，颈椎炎，滑囊炎，皮肤炎，结膜炎，中耳炎等等。
当炎症反应发生时，机体分泌大量抗炎症因子来激活免疫细胞如巨噬细胞、淋巴细胞、肥大细胞、T细胞、粒细胞，进入战斗状态，对致病菌进行杀伤，并且也可能进一步地释放促炎症因子，引发更为强烈的炎症反应。
RayBio提供多种产品供科研者研究炎症过程，各种炎症疾病发病机理，药物治疗炎症疾病的机制。
产品应用案例
案例1
杂志名称：Cell Research
影响因子：44.1（2023）
文献题目：Human ESC-derived expandable hepatic organoids enable therapeutic liver repopulation and pathophysiological modeling of alcoholic liver injury
本实验所用产品：AAH-INF-G3（半定量检测40个人类样本细胞因子）
实验样品：细胞上清
研究简介：在酒精性肝病治疗方案研究中，通过体外3D共培养模型，以及AAH-INF-G3 抗体芯片对hEHOs处理前后的炎症因子进行分析，发现hEHOs和人类胎儿肝间充质细胞（hFLMCs）生成的3D共培养肝类器官可如实地概括人类酒精性肝病（ALD）相关的病理生理学的各个方面，这可能是一个体外新的生物标志物筛选和新的治疗靶点探索ALD的机理模型。
研究思路1——hEHOs培养体系的建立
研究思路2——hEHOs表型鉴定
通过hEHOs的基因表达谱，转录组，形态学以及免疫荧光分析，发现hEHOs与肝脏谱系的新生阶段细胞非常相似，这反映在它们被赋予肝干/祖细胞表型。
hEHOs移植到非肥胖、糖尿病、严重联合免疫缺陷小鼠的附睾脂肪垫中，通过免疫染色分析hEHOs仅限于肝脏谱系，在所有移植hEHOs的小鼠中没有致癌潜力，并且可以分化成功能性肝细胞和胆管细胞。
研究思路3——hEHOs和人类胎儿肝间充质细胞（hFLMCs）体外3D共培养模型
体外3D共培养hEHOs和人类胎儿肝间充质细胞（hFLMCs）生成的3D共培养肝类器官利用AAH-INF-G3抗体芯片对hEHOs处理前后的炎症因子进行分析，发现可模拟人类酒精性肝病（ALD）的分子特性，病理结果相同，表明该模型可以作为ALD新的生物标志物筛选和新的治疗靶点探索的机理模型。
案例2
杂志名称：Nature Cell Biology
影响因子：21.3（2023）
文献题目：NAD+ metabolism governs the proinflammatory senescence-associated secretome
本实验所用产品：QAH-INF-3（定量检测40个人类样本炎症相关细胞因子）
实验样品：细胞上清
研究简介：在研究NAD+如何调节衰老相关分泌表型(SASP)的研究中，探究HMGA1调控的NAMPT在衰老过程中的潜在作用，通过敲低HMGA1或NAMPT或FK866抑制剂构建3组模型，用QAH-INF-3检测各组的炎症因子的表达，发现显著抑制SASP基因的促炎表达。HMGA1和NAMPT可以增强NAD+代谢，促进SASP，进而刺激肿瘤细胞的生长，同时为通过靶向NAD+治疗肿瘤提供了思路。
研究思路1——HMGA1与NAMPT基因表达的联系
对IMR90细胞的HMGA1染色质免疫共沉淀+二代测序（ChIP-seq）分析，发现NAMPT是HMGA1的靶基因，其表达量在衰老细胞中增加。
在癌基因诱导的衰老期间（ OIS ），HMGA1的下调抑制了NAMPT的上调，HMGA2敲低也抑制了NAMPT的上调。相反，异位HMGA1上调NAMPT表达。表明HMGA蛋白通过增强元件上调了OIS期间的NAMPT。
研究思路2——HMGA1与炎症反应的关系
探究HMGA1调控的NAMPT在衰老过程中的潜在作用，通过敲低HMGA1或NAMPT或FK866抑制剂构建3组模型，用QAH-INF-3检测各组的炎症因子的表达，发现显著抑制SASP基因的促炎表达。
研究思路3——NAD+调控SASP的促炎的机制研究
液质分析HMGA1或NAMPT处理后的各组的代谢物，发现NAD+/NADH比值的升高与NAMPT和促炎SASP的上调相吻合
用QAH-INF-3检测用NMN处理的癌细胞的炎症因子，显示NMN处理后的细胞炎症反应明显降低，同时抑制了AMPK和p38的活性，增加了p38和p65的活性，以及NF-κB启动子的活性。
结合western blot等实验验证，揭示了衰老细胞中，HMGA1和NAMPT可以增强NAD+代谢，促进SASP，进而刺激肿瘤细胞的生长，同时为通过靶向NAD+治疗肿瘤提供了思路。
其他产品炎症研究应用案例
初步统计，2023年RayBio科研产品支持炎症相关领域研究发表文章超400篇，累计影响因子近2500分，其中包含多篇Cell Research、SIGNAL TRANSDUCT TAR、ADVANCED MATERIALS、ANN RHEUM DIS等国内外高分期刊。
▼ RayBio®抗体芯片近期部分学术引用
| Year | Journal | 2023 IF | Article Title |
| 2017 | Nature Communications | 16.6 | Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis |
| 2017 | Nature Immunology | 30.5 | Direct control of regulatory T cells by keratinocytes |
| 2017 | Nature | 64.8 | Cytoplasmic chromatin triggers inflammation in senescence and cancer |
| 2017 | Nature Medicines | 82.9 | Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms |
| 2018 | Acta Biomaterialia | 9.7 | Manipulation of the response of human endothelial colony-forming cells by focal adhesion assembly using gradient nanopattern plates |
| 2018 | Journal for Immuno Therapy of Cancer | 10.9 | Randomized phase II trial of autologous dendritic cell vaccines versus autologous tumor cell vaccines in metastatic melanoma 5-year follow up and additional analyses |
| 2018 | Arthritis & Rheumatology | 13.3 | Myogenic Progenitor Cells Exhibit Type I Interferon–Driven Proangiogenic Properties and Molecular Signature During Juvenile Dermatomyositis |
| 2018 | Hepatology | 13.5 | Identification and Therapeutic Intervention of Coactivated Anaplastic Lymphoma Kinase, Fibroblast Growth Factor Receptor 2, and Ephrin Type-A Receptor 5 Kinases in Hepatocellular Carcinoma |
| 2018 | Hepatology | 13.5 | Sphingosine kinase 1 promotes liver fibrosis by preventing miR-19b-3p-mediated inhibition of CCR2 |
| 2018 | Cell Host & Microbe | 30.3 | Listeria Adhesion Protein Induces Intestinal Epithelial Barrier Dysfunction for Bacterial Translocation |
| 2018 | Cell | 64.5 | CD10+ GPR77+ cancer-associated fibroblasts promote cancer formation and chemoresistance by sustaining cancer stemness |
| 2019 | Theranostics | 12.4 | Monoclonal Antibody againstCXCL1 (HL2401) as a Novel Agent in Suppressing IL6 Expression and Tumoral Growth |
| 2019 | Neurology | 9.9 | Changes in cerebralautoregulation and blood biomarkers after remote ischemic preconditioning |
| 2019 | Nature Communications | 16.6 | Altered respiratory virome and serum cytokine profile associated with recurrent respiratory tract infections in children |
| 2019 | Nature Communications | 16.6 | Microenvironmental IL1β promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling |
| 2019 | Nature Cell Biology | 21.3 | NAD+ metabolism governs the proinflammatory senescence-associated secretome |
| 2019 | Nature Medicine | 82.9 | Stress–glucocorticoid–TSC22D3 axis compromises therapy-induced antitumor immunity |
| 2019 | Cell Research | 44.1 | Sodium oligomannate therapeutically remodels gutmicrobiota and suppresses gut bacterial amino acids-shaped neuroinflammation toinhibit Alzheimer’s disease progression |
| 2019 | Cell | 64.5 | Regional activation of myosin II in cancer cells drives tumor progression via a secretory cross-talk with the immune microenvironment |
| 2020 | Theranostics | 12.4 | Targeting the STING pathway in tumor-associated macrophages regulates innate immune sensing of gastric cancer cells |
| 2020 | Theranostics | 12.4 | Plasma protein-based signature predicts distant metastasis and inductionchemotherapy benefit in Nasopharyngeal Carcinoma |
| 2020 | Molecular Therapy | 12.4 | HLSC-Derived Extracellular Vesicles Attenuate Liver Fibrosis and Inflammation in a Murine Model of Non-alcoholic Steatohepatitis |
| 2020 | Acta Pharmaceutica Sinica B | 14.5 | Chrysin serves as a novel inhibitor of DGKa/FAK interaction to suppress the malignancy of esophageal squamous cellcarcinoma (ESCC) |
| 2020 | Journal of Extracellular Vesicles | 16 | Small extracellular vesicles derived from human mesenchymal stromal cells prevent group 2 innatelymphoid cell-dominant allergic airway inflammation through delivery ofmiR-146a-5p |
| 2020 | Nature Communications | 16.6 | Distinct effects of generalanesthetics on lung metastasis mediated by IL-6/JAK/STAT3 pathway in mouse models |
| 2020 | Molecular Cell | 16.6 | Ectosomal PKM2 Promotes HCC by Inducing Macrophage Differentiation and Remodeling the Tumor Microenvironment |
| 2020 | Immunity | 32.4 | Excessive polyamine generation in keratinocytes promotes self-RNA sensing by dendritic cells in psoriasis |
| 2021 | Pharmacological Research | 9.3 | Affiliated Hospital of Integrated Traditional Chinese and WesternMedicine, Nanjing University of Chinese Medicine |
| 2021 | Theranostics | 12.4 | Intravenous transplantationof olfactory ensheathing cells reduces neuroinflammation after spinal cordinjury via interleukin-1 receptor antagonist |
| 2021 | Theranostics | 12.4 | Tumor suppressor DRD2 facilitates M1 macrophages and restricts NF-κB signaling to trigger pyroptosis in breast cancer |
| 2021 | Hepatology | 13.5 | Group-2 Innate LymphoidCells Promote Hepatocellular Carcinoma Progression via CXCL2-Neutrophil Induced Immunosuppression |
| 2021 | Nature Cell Biology | 21.3 | m6A-independent genome-wide METTL3 and METTL14 redistribution drives the senescence-associated secretory phenotype |
| 2021 | Signal Transduction and Targeted Therapy | 39.3 | Mutant KRAS triggers functional reprogramming of tumor-associated macrophages in colorectal cancer |
| 2021 | Cancer Cell | 50.3 | Three subtypes of lung cancer fibroblasts define distinct therapeutic paradigms |
| 2022 | Journal for ImmunoTherapy of Cancer | 10.9 | Longitudinal plasma proteomic profiling of patients with non-small cell lung cancer undergoing immune checkpoint blockade |
| 2022 | PNAS | 11.1 | Plasma metabolome and cytokine profile reveal glycylproline modulating antibody fading in convalescent COVID-19 patients |
| 2022 | Advanced Science | 15.1 | Mouse Modeling Dissecting Macrophage–BreastCancer Communication Uncovered Roles of PYK2 in Macrophage Recruitment and Breast Tumorigenesis |
| 2022 | Nature Microbiology | 28.3 | Multi-omics analyses of airway host–microbe interactions in chronic obstructive pulmonary disease identify potential therapeutic interventions |
| 2023 | Nature Metabolism | 20.8 | TREM2hiresident macrophages protect the septic heart by maintaining cardiomyocyte homeostasis |
| 2023 | Journal of Hematology & Oncology | 28.5 | LCN2 secreted by tissue-infiltrating neutrophils induces the ferroptosis and wasting of adipose and muscle tissues in lung cancer cachexia |
| 2023 | Cancer Cell | 50.3 | Tissue factor is a critical regulator of radiation therapy-induced glioblastoma remodeling |
▼ RayBio®ELISA近期部分学术引用
| Year | Journal | 2023 IF | Article Title |
| 2017 | Science Translational Medicine | 17.1 | Detection of early pancreatic ductal adenocarcinoma using thrombospondin-2 and CA19-9 blood markers |
| 2017 | JAMA Internal Medicine | 39.0 | Association of Ozone Exposure With Cardiorespiratory Pathophysiologic Mechanisms in Healthy Adults |
| 2017 | Journal of Clinical Investigation | 15.9 | Thrombin promotes diet-induced obesity through fibrin-driven inflammation |
| 2017 | Nature Medicine | 82.9 | Targeting CASP8 and FADD-like apoptosis regulator ameliorates nonalcoholic steatohepatitis in mice and nonhuman primates |
| 2017 | Nature Communications | 16.6 | Tyrosine phosphatase SHP2 negatively regulates NLRP3 inflammasome activation via ANT1-dependent mitochondrial homeostasis |
| 2018 | Nature | 64.8 | Disruption of a self-amplifying catecholamine loop reduces cytokine release syndrome |
| 2018 | Autophagy | 13.3 | HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer |
| 2020 | Annals of Surgery | 9.0 | Plasma miR-181a-5p Downregulation Predicts Response and Improved Survival After FOLFIRINOX in Pancreatic Ductal Adenocarcinoma |
| 2020 | Nature Communications | 16.6 | Plasma levels of DPP4 activity and sDPP4 are dissociated from inflammation in mice and humans |
| 2020 | Nature Communications | 16.6 | Chemotaxis-driven delivery ofnano-pathogenoids for co mplete eradication oftumorspost-phototherapy |
| 2020 | Nature Communications | 16.6 | Receptor-targetedengineered probiotics mitigatele thal Listeria infection |
| 2020 | Brain Behavior and Immunity | 15.1 | Increased vulnerability to impulsive behavior after streptococcal antigen exposure and antibiotic treatment in rats |
| 2020 | Cell Metabolism | 29.0 | Defined p16High Senescent Cell Types Are Indispensablefor Mouse Healthspan |
| 2020 | Immunity | 32.4 | Interleukin-33 Promotes Serotonin Release from Enterochromaffin Cells for Intestinal Homeostasis |
| 2020 | Cell | 64.5 | Mortality Risk Profiling of Staphylococcus aureus Bacteremia by Multi-omic Serum Analysis Reveals Early Predictive and Pathogenic Signatures |
| 2021 | Journal of Clinical Investigation | 15.9 | Mutant SF3B1 promotes AKT-and NF-KB-driven mammary tumorigenesis |
| 2022 | Biomaterials | 14.0 | Dual-functionalhydrogel systemfor spinal cordr egeneration with sustained releaseofarylsulfataseBalleviatesfibrotic mi croenvironment and promotes axonalregeneration |
【蛋白芯片】专题集锦 | 炎症，自噬，肥胖，细胞周期，心血管，老年痴呆，外泌体，免疫，胰腺癌
【泪部资料】蛋白芯片案例集锦 | 肿瘤/癌症专题（一）科研重器15篇
【泪部资料】蛋白芯片案例集锦 | 免疫专题（一）6篇
【泪部资料】案例集锦 | 蛋白芯片与抗体芯片在生物医学研究和转化医学中的应用（一）
【泪部资料】案例集锦 | 蛋白芯片与抗体芯片在生物医学研究和转化医学中的应用（二）
【泪部资料】案例集锦 | 蛋白芯片与抗体芯片在生物医学研究和转化医学中的应用（三）
