Hippo信号通路
Hippo信号通路，也称为Salvador / Warts / Hippo(SWH)通路，主要参与器官发育中细胞增殖和凋亡的调控。
Hippo信号通路的一些关键因子包括MST1/2、SAV1、LATS1/2、MOB1、YAP/TAZ等参与MST-LATS级联反应的上游因子，结合TGFb通路关键因子（TGFb、Smad2/3、Smad7等）、Wnt信号通路关键因子（Wnt、DVL、14-3-3等），调控YAP/TAZ发挥主要作用，参与下游凋亡相关基因（CTGF、AREG、Id1等）表达，泛素化降解，细胞大小的调控等。
图片来自david数据库
Hippo信号通路的研究应用
Hippo信号通路的失调必然导致多种凋亡相关基因的表达失控，因此与多种癌症的发生、发展和转移密切相关，针对Hippo通路的靶向治疗策略作为肿瘤治疗的重要关注点。
Hippo通路通过调控细胞增殖和细胞外基质的产生，影响纤维化的进程，已经有大量研究表明，Hippo通路与肝、肺、肾等器官、组织等[1,2,3]细胞的纤维化相关, 因此调控Hippo通路可能成为治疗纤维化疾病的潜在策略。
除了细胞发育，Hippo也参与骨骼机械应力的调控，研究表明，通过调控Hippo通路中的因子可能成为干预骨关节炎的新靶点，为骨关节炎的发病机制的挖掘及疾病防控提供思路[4]。
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在疾病研究中如何快速检测实验样本Hippo通路的所有靶标信息？
Empowering your proteomics
RayBio Hippo通路专用检测试剂盒，仅需60μg总蛋白，即可检测胞内外Hippo通路118个上下游核心蛋白表达水平，精准、高效、全面！
货号：AAH-BLG-HIP
检测因子数量：118（细胞内外Hippo通路功能性细胞因子）
技术原理：抗原抗体结合，生物素标记法，半定量
可选载体：载玻片
检测样本：人类细胞、组织裂解液
检测仪器：玻璃芯片扫描仪（Cy3荧光、分辨率≤20μm）
优势：自带2个技术重复，微量样本多因子检测，抗原抗体结合灵敏度高！
产品应用
本产品适用于Hippo通路相关的分子机制深度挖掘，癌症、纤维化等疾病相关发病机制、治疗、药物作用等相关研究，具体如下：
①Hippo通路核心蛋白相对表达量检测;
②Hippo、TGFb、Wnt等多通路互作探索；
③细胞因子表达模式的检测生物标志物的筛选;
④疾病及药物作用标志物筛选；
⑤Hippo相关分子作用信息补充；
⑥细胞凋亡、细胞增殖、泛素化等调控机制探索；
参考文献
[1] Song, Jiagui et al. “Kindlin-2 Inhibits the Hippo Signaling Pathway by Promoting Degradation of MOB1.” Cell reports vol. 29,11 (2019): 3664-3677.e5. doi:10.1016/j.celrep.2019.11.035
[2]Shen, Hongyu et al. “The Hippo pathway links adipocyte plasticity to adipose tissue fibrosis.” Nature communications vol. 13,1 6030. 13 Oct. 2022, doi:10.1038/s41467-022-33800-0
[3]ZHAO XL, ZHANG CY, GAO XY, et al. Mechanism of action of the Hippo/YAP pathway in the development and progression of liver fibrosis[J]. J Clin Hepatol, 2022, 38(7): 1654-1657. DOI: 10.3969/j.issn.1001-5256.2022.07.037.
[4]杨攀, et al."机械应力调控Hippo通路影响骨关节炎的发生与发展."中国组织工程研究 28.30(2024):4902-4908.
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