摘要：沙棘（Hippophae rhamnoides L.）作为一种常见的药食同源植物，具有较高营养价值和生物活性，常分布于我国西北、东北等地。沙棘多糖（polysaccharide from Hippophae rhamnoides，PBP）作为沙棘中最主要的活性成分具有抗氧化、抗疲劳、抑制肿瘤、降低血糖等药用价值。运动性疲劳会使机体产生大量的自由基和过氧化物，而抗氧化能力同样也会影响抗疲劳的效果。因此，本文主要研究沙棘多糖对长时间过量运动小鼠体力疲劳的缓解作用。小鼠分为空白组、PBP 低（100 mg/kg）、中（200 mg/kg）、高（400 mg/kg）剂量组，连续灌胃 15 d，记录力竭游泳时间，测定血乳酸（lactic acid，LA）、血清尿素氮（blood urea nitrogen，BUN）、肝脏中肝糖原（hepaticr glycogen，HG）含量，以及体外 DPPH 自由基、ABTS+自由基清除率和总还原能力。结果表明，与空白对照组相比，PBP 能够显著延长小鼠负重游泳时间（P<0.05），其中中剂量组游泳时间增加极显著（P<0.01）。PBP 中、高剂量组极显著（P<0.01）的降低了血清中 BUN、LA 含量，中剂量组极显著（P<0.01）的增加了肝脏中 HG 的储存量。体外抗氧化结果表明，沙棘多糖对 DPPH 自由基、ABTS+自由基都具有一定的清除能力，其IC50值分别为 0.81、2 mg/mL，当浓度为 1 mg/mL 时，总还原能力为 0.727。结果表明沙棘多糖具有一定的缓解体力疲劳、增强小鼠运动能力和抗氧化的作用。
图片来源于图司机
Abstract: Hippophae rhamnoides as a common medicine-food homologous plant, has high nutritional value and biological activity, and is often distributed in northwest and northeast China. Polysaccharide from Hippophae rhamnoides (PBP) , as the most important active ingredient in Hippophae rhamnoides, has the medicinal value of anti-oxidation, anti-fatigue, anti-tumor, reducing blood glucose and so on. Exercise fatigue would cause the body to produce a large number of free radicals and peroxide, meanwhile antioxidant capacity will also affect the effect of anti-fatigue. Therefore, this paper mainly studied the effect of polysaccharide from Hippophae rhamnoides on mitigating physical fatigue in mice with prolonged excessive exercise. The mice were divided into blank group, low (100 mg/kg), middle (200 mg/kg) and high (400 mg/kg) dose group of PBP, and fed continuous gavage for 15 d. Exhaustive swimming time was recorded. Then, the levels of blood lactic acid (LA), blood urea nitrogen (BUN) and the contents of glycogen. The results showed that, compared with the blank group, PBP significantly prolonged exhaustive swimming time (P<0.05). Exhaustive swimming time in middle PBP group increased significantly (P<0.01). And it significantly decreased the levels of BUN and LA in middle and high PBP groups. The level of HG in middle PBP group increased significantly (P<0.01). The antioxidant activity experiment in vitro showed that, the polysaccharide of Hippophae rhamnoides had certain scavenging ability to DPPH and ABTS+ free radical. And the level of IC50 was 0.81 and 2 mg/mL. When the concentration of polysaccharide of Hippophae rhamnoides was 1 mg/mL, the total reducing power was 0.727. Therefore, the polysaccharide of Hippophae rhamnoides can relieve physical fatigue, enhance the exercise ability of mice and have effect on antioxidant.
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