在2017年1月5日Cell Chemical Biology.期刊中由美国密歇根大学Jeannie L. Hernandez, Dahvid Davda等为第一作者，发表了一篇《APT2 Inhibition Restores Scribble Localization and S-Palmitoylation in Snail-Transformed Cells》的文章。
文章中提到多结构域支架蛋白Scribble (Scrib)是组织关键的信号复合体用以调控基底细胞的极性和抑制异常畸形生长。在人类癌症中，正常表达的Scrib通过细胞和细胞的连接到达细胞质基质，与增强生长的信号和恶性肿瘤相关。我们确认上皮细胞-间充质转录因子（EMT-TF）Snail在良性上皮细胞中的表达导致Scrib从质膜中位移，模拟了浸润性癌症中的错位移动。经过进一步的研究，Snail促进棕榈酰化循环中靶基因的转录，此转录程序抑制众多蛋白酰基转移酶, 提升蛋白酰基硫脂酶2（APT2）的表达和活性。当MEK活性减弱的时候，APT2亚型选择性的抑制或者降低活化Scrib膜定位和棕榈酰化。总体而言，抑制ATP2，使Scrib恢复到蛋白棕榈循环平衡上来，促进膜定位和生长衰减。这些发现强调S-棕榈酰化作为细胞极性介导的肿瘤抑制的翻译后关键者的重要性。
文中使用Azure biosystems near-700和near-800标记的近红外抗体，使用AzureC600多功能分子成像系统双激光近红外模块进行免疫印迹成像和精准定量分析，为后续实验奠定实验基础。
原文摘要：
The multidomain scaffolding protein Scribble (Scrib) organizes key signaling complexes to specify baso-lateral cell polarity and suppress aberrant growth. In many human cancers, genetically normal Scrib mislocalizes from cell-cell junctions to the cytosol, correlating with enhanced growth signaling and malignancy. Here we confirm that expression of the epithelial-to-mesenchymal transcription factor (EMT-TF) Snail in benign epithelial cells leads to Scrib displacement from the plasma membrane, mimicking the mislocalization observed in aggres-sive cancers. Upon further examination, Snail pro-motes a transcriptional program that targets genes in the palmitoylation cycle, repressing many protein acyl transferases and elevating expression and activity of protein acyl thioesterase 2 (APT2). APT2 isoform-selective inhibition or knockdown rescued Scrib membrane localization and palmitoylation while attenuating MEK activation. Overall, inhibiting APT2 restores balance to the Scrib palmitoylation cy-cle, promoting membrane re-localization and growth attenuation. These findings emphasize the impor-tance of S-palmitoylation as a post-translational gatekeeper of cell polarity-mediated tumor sup-pression.
Azure Biosystems的c600多功能分子成像系统，同一台设备即可满足核酸胶、蛋白胶、化学发光Western Blot和近红外荧光Western Blot的应用需求。
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